Evidence map›Paper›PMID 34430333›Full record

ArticleMethodsX2021

Transient expression of fluorescently tagged proteins in developing maize aleurone cells.

Xinxin Ding, Xiaoguo Zhang, Odd-Arne Olsen, Marisa S Otegui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Xinxin DingDepartment of Botany and Center for Quantitative Cell Imaging, University of Wisconsin-Madison, WI, United States.
Xiaoguo ZhangDepartment of Botany and Center for Quantitative Cell Imaging, University of Wisconsin-Madison, WI, United States.
Odd-Arne OlsenDepartment of Plant Science, Norwegian University of Life Sciences, Ås 1434, Norway.
Marisa S OteguiDepartment of Botany and Center for Quantitative Cell Imaging, University of Wisconsin-Madison, WI, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Expressing transgenes in the endosperm of cereals by developing stably transformed lines is an expensive and labor-intensive process. An alternative that is less expensive and faster is to express the transgenes transiently. We describe here a detailed protocol to express transiently genes in maize aleurone cells by biolistic bombardment of

Indexed as

Biolistic bombardmentCo-expression of GFP and mCherryIn vitro endosperm culture

Identifiers

PMID34430333
PMCPMC8374648

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.