ArticleThe ISME journal2022
Genetic engineering of marine cyanophages reveals integration but not lysogeny in T7-like cyanophages.
Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Genetic engineering of cyanophages for harmful cyanobacterial bloom treatment-A mini review.Synthetic and systems biotechnology · 2027Review
- Genetic Engineering of Lysogenic-Lytic Switch Genes ImprovesInternational journal of molecular sciences · 2026Article
- A pentose, as a cytosine nucleobase modification inApplied and environmental microbiology · 2026Article
- Unveiling a novel broad-host-range cyanomyovirus cross-infecting Prochlorococcus and Synechococcus.BMC biology · 2025Article
- Seasonal enhancement of the viral shunt catalyzes a subsurface oxygen maximum in the Sargasso Sea.Nature communications · 2025Article
- Article
- CRISPR-Cas9 enables efficient genome engineering of the strictly lytic, broad-host-range staphylococcal bacteriophage K.Applied and environmental microbiology · 2025Article
- Marine Autographiviridae phages exhibit high genetic diversity and global-scale biogeographic patterns.Communications biology · 2025Article
- Marine Bacteriophages as Next-Generation Therapeutics: Insights into Antimicrobial Potential and Application.Viruses · 2025Review
- Adaptive loss of tRNA gene expression leads to phage resistance in a marine Synechococcus cyanobacterium.Nature microbiology · 2025Article
- Engineered Bacteriophages: Advances in Phage Genome Redesign Strategies for Therapeutic and Environmental Applications.Protein and peptide letters · 2025Review
- Cyanophage Engineering for Algal Blooms Control.Viruses · 2024Review
- Efficient Broad-Spectrum Cyanophage Function Module Mining.Microorganisms · 2024Article
- Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia.Marine drugs · 2024Review
- Characterization and genomic analysis of a novel Synechococcus phage S-H9-2 belonging to Bristolvirus genus isolated from the Yellow Sea.Virus research · 2023Article
- Light and carbon: Synthetic biology toward new cyanobacteria-based living biomaterials.Materials today. Bio · 2023Article
- Review
- Abundant and cosmopolitan lineage of cyanopodoviruses lacking a DNA polymerase gene.The ISME journal · 2023Article
- Marine viral particles reveal an expansive repertoire of phage-parasitizing mobile elements.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- PhageLeads: Rapid Assessment of Phage Therapeutic Suitability Using an Ensemble Machine Learning Approach.Viruses · 2022Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine cyanobacteria of the genera Synechococcus and Prochlorococcus are the most abundant photosynthetic organisms on earth, spanning vast regions of the oceans and contributing significantly to global primary production. Their viruses (cyanophages) greatly influence cyanobacterial ecology and evolution. Although many cyanophage genomes have been sequenced, insight into the functional role of cyanophage genes is limited by the lack of a cyanophage genetic engineering system. Here, we describe a simple, generalizable method for genetic engineering of cyanophages from multiple families, that we named REEP for REcombination, Enrichment and PCR screening. This method enables direct investigation of key cyanophage genes, and its simplicity makes it adaptable to other ecologically relevant host-virus systems. T7-like cyanophages often carry integrase genes and attachment sites, yet exhibit lytic infection dynamics. Here, using REEP, we investigated their ability to integrate and maintain a lysogenic life cycle. We found that these cyanophages integrate into the host genome and that the integrase and attachment site are required for integration. However, stable lysogens did not form. The frequency of integration was found to be low in both lab cultures and the oceans. These findings suggest that T7-like cyanophage integration is transient and is not part of a classical lysogenic cycle.
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