Evidence map›Paper›PMID 34429488›Full record

ArticleScientific reports2021

Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR

Plinio Trabasso, Tetsuhiro Matsuzawa, Teppei Arai, Daisuke Hagiwara, Yuzuru Mikami, Maria Luiza Moretti, Akira Watanabe

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Rapid and Cost-EffectiveDiagnostics (Basel, Switzerland) · 2025
    Article
  4. Review
  5. Review
  6. Insights intoJournal of fungi (Basel, Switzerland) · 2024
    Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Plinio TrabassoSchool of Medical Sciences, University of Campinas, Campinas, Sao Paulo, Brazil. trabasso@unicamp.br.ORCID 0000-0002-0588-4859
Tetsuhiro MatsuzawaUniversity of Nagasaki, Nagasaki, Japan.
Teppei AraiMedical Mycology Research Center, Chiba University, Chiba, Japan.
Daisuke HagiwaraMedical Mycology Research Center, Chiba University, Chiba, Japan.
Yuzuru MikamiMedical Mycology Research Center, Chiba University, Chiba, Japan.
Maria Luiza MorettiSchool of Medical Sciences, University of Campinas, Campinas, Sao Paulo, Brazil.
Akira WatanabeMedical Mycology Research Center, Chiba University, Chiba, Japan.
Chiba University Hospital · JPUniversidade Estadual de Campinas (UNICAMP) · BRUniversity of Nagasaki · JPUniversity of Tsukuba · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Infections due to triazole-resistant Aspergillus fumigatus are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34 bp or 46 bp within the promoter region of the cyp51A gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46 bp tandem repeat insertion in the cyp51A gene promoter region based on novel LAMP primer sets. It also differentiated strains with TR

Indexed as

Drug Resistance, FungalAntifungal AgentsAspergillus fumigatusAzolesCytochrome P-450 Enzyme SystemDNA PrimersFungal ProteinsMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPromoter Regions, GeneticTandem Repeat SequencesAntifungal AgentsAzolescytochrome P-450 CYP51A, AspergillusCytochrome P-450 Enzyme SystemDNA PrimersFungal Proteins

Identifiers

PMID34429488
PMCPMC8384855
OpenAlexW3134424643

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.