ArticleBMC biology2021
Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology.
Article in BMC biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
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- Genome editing across Dictyostelia species enables comparative functional genetics of social amoebas.Scientific reports · 2026Article
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- Cell biology of Dictyostelium.BMC molecular and cell biology · 2025Article
- Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase iniScience · 2024Article
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- Functional mechanism study of the allelochemical myrigalone A identifies a group of ultrapotent inhibitors of ethylene biosynthesis in plants.Plant communications · 2024Article
- Mutation Screening of Dictyostelium Restriction Enzyme-Mediated Integration (REMI) Libraries.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Studies of Streptococcus anginosus Virulence in Dictyostelium discoideum and Galleria mellonella Models.Infection and immunity · 2023Article
- Spindly is a nucleocytosolic O-fucosyltransferase in Dictyostelium and related proteins are widespread in protists and bacteria.Glycobiology · 2023Article
- Synergy between a cytoplasmic vWFA/VIT protein and a WD40-repeat F-box protein controls development inFrontiers in cell and developmental biology · 2023Article
- Oxygen-dependent regulation of E3(SCF)ubiquitin ligases and a Skp1-associated JmjD6 homolog in development of the social amoeba Dictyostelium.The Journal of biological chemistry · 2022Article
- CRISPR/Cas9-based genome-wide screening of Dictyostelium.Scientific reports · 2022Article
- Review
- The genetic architecture underlying prey-dependent performance in a microbial predator.Nature communications · 2022Article
- Mfsd8 Modulates Growth and the Early Stages of Multicellular Development inFrontiers in cell and developmental biology · 2022Article
- Moving the Research Forward: The Best of British Biology Using the Tractable Model SystemCells · 2021Review
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundGenomes can be sequenced with relative ease, but ascribing gene function remains a major challenge. Genetically tractable model systems are crucial to meet this challenge. One powerful model is the social amoeba Dictyostelium discoideum, a eukaryotic microbe widely used to study diverse questions in the cell, developmental and evolutionary biology.
resultsWe describe REMI-seq, an adaptation of Tn-seq, which allows high throughput, en masse, and quantitative identification of the genomic site of insertion of a drug resistance marker after restriction enzyme-mediated integration. We use REMI-seq to develop tools which greatly enhance the efficiency with which the sequence, transcriptome or proteome variation can be linked to phenotype in D. discoideum. These comprise (1) a near genome-wide resource of individual mutants and (2) a defined pool of 'barcoded' mutants to allow large-scale parallel phenotypic analyses. These resources are freely available and easily accessible through the REMI-seq website that also provides comprehensive guidance and pipelines for data analysis. We demonstrate that integrating these resources allows novel regulators of cell migration, phagocytosis and macropinocytosis to be rapidly identified.
conclusionsWe present methods and resources, generated using REMI-seq, for high throughput gene function analysis in a key model system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.