Evidence map›Paper›PMID 34428454›Full record

ArticleMolecular cell2021

Interrogation of the dynamic properties of higher-order heterochromatin using CRISPR-dCas9.

Yuchen Gao, Mengting Han, Stephen Shang, Haifeng Wang, Lei S Qi

Open access · greenAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 32 citations in OpenAlex.

  1. 3D Genome Engineering Using CRISPR/dCas Systems.International journal of molecular sciences · 2026
    Review
  2. Article
  3. Article
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  5. Article
  6. Review
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
  12. HP1-driven phase separation recapitulates the thermodynamics and kinetics of heterochromatin condensate formation.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  13. Review
  14. Article
  15. Review
  16. Engineered PROTAC-CID Systems for Mammalian Inducible Gene Regulation.Journal of the American Chemical Society · 2023
    Article
  17. Review
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yuchen GaoCancer Biology Program, Stanford University, Stanford, CA 94305, USA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Mengting HanDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Stephen ShangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Haifeng WangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Lei S QiDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; ChEM-H, Stanford University, Stanford, CA 94305, USA. Electronic address: stanley.qi@stanford.edu.
Stanford University · US

Funding

Engineering and Imaging 3D genome structure-function dynamics across time scalesU01DK127405 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI BLOBEL, GERD A, PHILLIPS-CREMINS, JENNIFER ELIZABETH · 2020 to 2024
$5.7M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01EB021240 · NIBIB · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2015 to 2017
$1.4M
NIBIB NIH HHS U01 EB021240NIDDK NIH HHS U01 DK127405
6 · The paper itself

Abstract

Eukaryotic chromosomes feature large regions of compact, repressed heterochromatin hallmarked by Heterochromatin Protein 1 (HP1). HP1 proteins play multi-faceted roles in shaping heterochromatin, and in cells, HP1 tethering to individual gene promoters leads to epigenetic modifications and silencing. However, emergent properties of HP1 at supranucleosomal scales remain difficult to study in cells because of a lack of appropriate tools. Here, we develop CRISPR-engineered chromatin organization (EChO), combining live-cell CRISPR imaging with inducible large-scale recruitment of chromatin proteins to native genomic targets. We demonstrate that human HP1α tiled across kilobase-scale genomic DNA form novel contacts with natural heterochromatin, integrates two distantly targeted regions, and reversibly changes chromatin from a diffuse to compact state. The compact state exhibits delayed disassembly kinetics and represses transcription across over 600 kb. These findings support a polymer model of HP1α-mediated chromatin regulation and highlight the utility of CRISPR-EChO in studying supranucleosomal chromatin organization in living cells.

Indexed as

Chromatin Assembly and DisassemblyClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsCell Line, TumorChromobox Protein Homolog 5CRISPR-Associated Protein 9HEK293 CellsHeterochromatinHumansNucleic Acid ConformationProtein ConformationRNA, Guide, CRISPR-Cas SystemsStructure-Activity RelationshipTime FactorsCas9 endonuclease Streptococcus pyogenesCBX5 protein, humanChromobox Protein Homolog 5CRISPR-Associated Protein 9HeterochromatinRNA, Guide, CRISPR-Cas Systems3D genomeCas9chromatin conformationCRISPRCRISPR-EChOepigeneticsgene regulationheterochromatinHP1synthetic biology

Identifiers

PMID34428454
PMCPMC8541924
OpenAlexW3195682473

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.