ArticlePLoS biology2021
Unique properties of dually innervated dendritic spines in pyramidal neurons of the somatosensory cortex uncovered by 3D correlative light and electron microscopy.
Article in PLoS biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Clustered inputs engage dendritic nonlinearities and calcium signaling to support efficient place-field formation in CA1 pyramidal neurons.Cell reports · 2026Article
- Review
- Subcellular Structures in Native Hippocampal Synapses Revealed by Cryo-electron Tomography.Neuroscience bulletin · 2026Article
- Spine-neck electrical bottlenecks tune temporal precision and inhibitory gating in cortical pyramidal neurons: A connectomics-based biophysical study.bioRxiv : the preprint server for biology · 2026Article
- 3D dendritic spines shape descriptors for efficient classification and morphology analysis in control and Alzheimer's disease modeling neurons.Bioinformatics (Oxford, England) · 2026Article
- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microscopy Imaging of Single Stimulated Synapses.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Widespread co-release of glutamate and GABA throughout the mouse brain.Communications biology · 2024Article
- Fiber-basedNeurophotonics · 2024Article
- Induction of LTP mechanisms in dually innervated dendritic spines.Scientific reports · 2024Article
- Tracing nerve fibers with volume electron microscopy to quantitatively analyze brain connectivity.Communications biology · 2024Article
- Enhanced Spine Stability and Survival Lead to Increases in Dendritic Spine Density as an Early Response to Local Alpha-Synuclein Overexpression in Mouse Prefrontal Cortex.Cellular and molecular neurobiology · 2024Article
- Prevalent co-release of glutamate and GABA throughout the mouse brain.bioRxiv : the preprint server for biology · 2024Article
- Article
- Three-dimensional analysis of interstitial cells in the lamina propria of the murine vas deferens by confocal laser scanning microscopy and FIB/SEM.Scientific reports · 2022Article
- The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses.PLoS biology · 2022Article
- Inhibitory postsynaptic density from the lens of phase separation.Oxford open neuroscience · 2022Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyramidal neurons (PNs) are covered by thousands of dendritic spines receiving excitatory synaptic inputs. The ultrastructure of dendritic spines shapes signal compartmentalization, but ultrastructural diversity is rarely taken into account in computational models of synaptic integration. Here, we developed a 3D correlative light-electron microscopy (3D-CLEM) approach allowing the analysis of specific populations of synapses in genetically defined neuronal types in intact brain circuits. We used it to reconstruct segments of basal dendrites of layer 2/3 PNs of adult mouse somatosensory cortex and quantify spine ultrastructural diversity. We found that 10% of spines were dually innervated and 38% of inhibitory synapses localized to spines. Using our morphometric data to constrain a model of synaptic signal compartmentalization, we assessed the impact of spinous versus dendritic shaft inhibition. Our results indicate that spinous inhibition is locally more efficient than shaft inhibition and that it can decouple voltage and calcium signaling, potentially impacting synaptic plasticity.
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