ArticlePhysiological reports2021
High-throughput determination of oxygen dissociation curves in a microplate reader-A novel, quantitative approach.
Article in Physiological reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Effects of the micronutrient Sanopal® (5-hydroxymethyl-2-furfural and α-ketoglutaric acid) on oxygen affinity of hemoglobin, oxygen saturation and exercise responses at altitude.Journal of the International Society of Sports Nutrition · 2026Trial
- A new approach to haemoglobin oxygen affinity research at high altitude: Determination of haemoglobin oxygen dissociation curves and 2,3-bisphosphoglycerate in an experimental human crossover hypoxic chamber study.European journal of applied physiology · 2025Trial
- The effect of desflurane, isoflurane and sevoflurane on the hemoglobin oxygen dissociation curve in human blood samples.Scientific reports · 2022Trial
- Dissolved Oxygen-Dependent Magnetophoresis of Sickle Red Blood Cells and Proof-of-Concept Separation Using a Magnetic Column.ACS measurement science au · 2026Article
- Inflammation and Hemoglobin Oxygen Affinity.Inflammation · 2025Article
- Advances in the characterization of in vitro-generated red blood cells: from biophysical properties to functional applications.Stem cell research & therapy · 2025Review
- Effects of drugs on the oxygen dissociation curve-a scoping review.European journal of clinical pharmacology · 2025Article
- Rapid measurement of hemoglobin-oxygen dissociation by leveraging Bohr effect and Soret band bathochromic shift.The Analyst · 2024Article
- The oxygen dissociation curve of blood in COVID-19-An update.Frontiers in medicine · 2023Review
- The impact of nebulized epoprostenol and iloprost on hemoglobin oxygen affinity: an ex vivo experiment.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Dose- and Sex-Dependent Changes in Hemoglobin Oxygen Affinity by the Micronutrient 5-Hydroxymethylfurfural and α-Ketoglutaric Acid.Nutrients · 2021Article
- High-throughput determination of oxygen dissociation curves in a microplate reader-A novel, quantitative approach.Physiological reports · 2021Article
- Effects of Carbon Dioxide and Temperature on the Oxygen-Hemoglobin Dissociation Curve of Human Blood: Implications for Avalanche Victims.Frontiers in medicine · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro determination of the hemoglobin oxygen dissociation curve (ODC) requires highly elaborate, specialized, and costly technical equipment. In addition, there is a lack of methods that combine reliable ODC recordings with high throughput in small blood samples for routine analysis. We here introduce a modified, commercial 96-well plate with an integrated unidirectional gas flow system specifically adapted for use in fluorescence microplate readers. Up to 92 samples of whole or hemolyzed, buffered or unbuffered blood, including appropriate controls or internal standard hemoglobin solutions, can be analyzed within ~25 min. Oxygen saturation is measured in each well with dual wavelength spectroscopy, and oxygen partial pressure using fluorescence lifetime of commercial oxygen sensors at the in- and outlet ports of the gas-flow system. Precision and accuracy of this method have been determined and were compared with those of a standard method. We further present two applications that exemplarily highlight the usefulness and impact of this novel approach for clinical diagnostics or basic research.
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