ArticleMolecular plant pathology2021
A novel robust and high-throughput method to measure cell death in Nicotiana benthamiana leaves by fluorescence imaging.
Article in Molecular plant pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Upcycling Industrial Lignin Into Cationic Hydrogels With Potential for Sustainable Plant Protection.ChemSusChem · 2026Article
- NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana.The New phytologist · 2026Article
- Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter.Horticulture research · 2026Article
- Dissecting the causal polymorphism of the Lr67res multipathogen resistance gene.Journal of experimental botany · 2024Article
- Parallel, Continuous Monitoring and Quantification of Programmed Cell Death in Plant Tissue.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Type II metacaspase mediates light-dependent programmed cell death in Chlamydomonas reinhardtii.Plant physiology · 2024Article
- Highlighter: An optogenetic system for high-resolution gene expression control in plants.PLoS biology · 2023Article
- A conserved microtubule-binding region in Xanthomonas XopL is indispensable for induced plant cell death reactions.PLoS pathogens · 2023Article
- The activity of the RGA5 sensor NLR from rice requires binding of its integrated HMA domain to effectors but not HMA domain self-interaction.Molecular plant pathology · 2022Article
- New recognition specificity in a plant immune receptor by molecular engineering of its integrated domain.Nature communications · 2022Article
- A novel robust and high-throughput method to measure cell death in Nicotiana benthamiana leaves by fluorescence imaging.Molecular plant pathology · 2021Article
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4 authors.
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Abstract
Assessing immune responses and cell death in Nicotiana benthamiana leaf agro-infiltration assays is a powerful and widely used experimental approach in molecular plant pathology. Here, we describe a reliable high-throughput protocol to quantify strong, macroscopically visible cell death responses in N. benthamiana agro-infiltration assays. The method relies on measuring the reduction of leaf autofluorescence in the red spectrum upon cell death induction and provides quantitative data suitable for straightforward statistical analysis. Two different well-established model nucleotide-binding and leucine-rich repeat domain proteins (NLRs) were used to ensure the genericity of the approach. Its accuracy and versatility were compared to visual scoring of the cell death response and standard methods commonly used to characterize NLR activities in N. benthamiana. A discussion of the advantages and limitations of our method compared to other protocols demonstrates its robustness and versatility and provides an effective means to select the best-suited protocol for a defined experiment.
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