Evidence map›Paper›PMID 34426961›Full record

ReviewAdvances in experimental medicine and biology2022

In Vitro Culturing of Adult Stem Cells: The Importance of Serum and Atmospheric Oxygen.

Maša Čater, Gregor Majdič

Abstract readReview
PubMed Publisher
In one paragraph

Review in Advances in experimental medicine and biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Maša ČaterLaboratory for Animal Genomics, Institute for Preclinical Studies, Veterinary faculty, University of Ljubljana, Ljubljana, Slovenia.
Gregor MajdičLaboratory for Animal Genomics, Institute for Preclinical Studies, Veterinary faculty, University of Ljubljana, Ljubljana, Slovenia. gregor.majdic@vf.uni-lj.si.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adult stem cells are undifferentiated cells found in many different tissues in the adult human and animal body and are thought to be important for replacing damaged and dead cells during life. Due to their differentiation abilities, they have significant potential for regeneration and consequently therapeutic potential in various medical conditions. Studies on in vitro cultivation of different types of adult stem cells have shown that they have specific requirements for optimal proliferation and stemness maintenance as well as induced differentiation. The main factors affecting the success of stem cell cultivation are the composition of the growth medium, including the presence of serum, temperature, humidity, and contact with other cells and the composition of the atmosphere in which the cells grow. In this chapter, we review the literature and describe our own experience regarding the influence of the presence of fetal bovine serum in the medium and the oxygen concentration in the atmosphere on the stemness maintenance and survival of adult stem cells from various tissue sources such as adipose tissue, muscle, brain, and testicular tissue.

Indexed as

Adult Stem CellsOxygenAdipose TissueAnimalsCell DifferentiationCell ProliferationCells, CulturedCulture MediaStem CellsCulture MediaOxygenAdipose tissueAtmospheric oxygenBrainCulturingDifferentiationMuscleSerumStem cellsStemnessTestes

Identifiers

PMID34426961

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.