ArticleMolecular diagnosis & therapy2021
Diagnostic Accuracy of Two Molecular Tools for Diagnosis of Congenital Chagas Disease.
Article in Molecular diagnosis & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Molecular diagnosis of Chagas disease: a systematic review and meta-analysis.Infectious diseases of poverty · 2023Pooled it
- Towards new care strategies in a suburban healthcare center in La Matanza, Argentina: performance evaluation of rapid diagnostic tests for Chagas disease in a non-endemic area.Frontiers in cellular and infection microbiology · 2026Article
- A specific, stable, and accessible LAMP assay targeting the HSP70 gene ofMicrobiology spectrum · 2025Article
- Detection ofTropical medicine and infectious disease · 2025Article
- Diagnosing Chagas in Pregnancy and Childhood: What's Old and New.Clinics in laboratory medicine · 2025Review
- Congenital Chagas disease: A cohort study to assess molecular diagnostic methods at the Chagas disease national reference center of Argentina.PLoS neglected tropical diseases · 2025Article
- Development of a Colorimetric Loop-Mediated Isothermal Amplification Assay for the Detection ofDiagnostics (Basel, Switzerland) · 2024Article
- Article
- Efficacy of short-course treatment for prevention of congenital transmission of Chagas disease: A retrospective cohort study.PLoS neglected tropical diseases · 2024Article
- Subunit nanovaccine elicited T cell functional activation controls Trypanosoma cruzi mediated maternal and placental tissue damage and improves pregnancy outcomes in mice.NPJ vaccines · 2023Article
- Effects of Acute and Chronic Trypanosoma cruzi Infection on Pregnancy Outcomes in Mice: Parasite Transmission, Mortality, Delayed Growth, and Organ Damage in Pups.The American journal of pathology · 2023Article
- Clinical use of molecular methods forFrontiers in parasitology · 2023Article
- Laboratory diagnosis ofFrontiers in parasitology · 2023Review
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Authors and funding
10 authors.
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Abstract
BACKGROUND AND
objectiveThe real prevalence of congenital Chagas disease is undefined because of difficulties in the detection of Trypanosoma cruzi by microscopic examination. The aim of this study was to determine the diagnostic accuracy of two molecular diagnostic tools, qPCR and LAMP, in the diagnosis of congenital Chagas disease in a clinical setting.
methodsTo this end, we conducted a prospective cohort study in a tertiary care center, of infants under 9 months of age, born in Buenos Aires to women with Chagas disease. Blood samples were collected for microscopic examination and molecular diagnosis at baseline. If negative, infants were followed up until 9 months of age to determine a final diagnosis by serology. In-house qPCR and LAMP previously validated were challenged as index tests.
resultsA total of 154 participants were potentially eligible, 120 of whom were enrolled. Finally, 102 (66.2%) of them fulfilled the follow-up. The diagnosis of congenital Chagas disease was confirmed in 13 infants and excluded in 89. Both the sensitivity and specificity of the qPCR were 100.0% (95% confidence interval 75.3-100.0 and 95% confidence interval 95.9-100.0, respectively), whereas the sensitivity and specificity of LAMP were 69.2% (95% confidence interval 38.6-90.9) and 100% (95% confidence interval 95.9-100.0), respectively.
conclusionsThe qPCR agreed with the current diagnostic algorithm, and was a reliable and sensitive tool to detect congenital Chagas disease earlier, providing an appropriate and timely identification of infected infants requiring treatment. LAMP was able to detect congenital Chagas disease in infected infants by naked-eye visualization in accordance with a microscopic examination. The advantages of molecular diagnostic tools should be taken into account by the health system to improve congenital Chagas disease diagnosis.
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