ArticleCommunications biology2021
Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Metagenome Sequencing and Recovery of 52 Microbial Genomes from Plastic-Polluted Coastal Sediment.Scientific data · 2026Article
- The pangenome: a statistical model, not a fixed biological property.Bioinformatics advances · 2026Review
- Genome-resolved long-read sequencing expands known microbial diversity across terrestrial habitats.Nature microbiology · 2025Article
- Revealing microbial consortia that interfere with grapevine downy mildew through microbiome epidemiology.Environmental microbiome · 2025Article
- Metagenomics as a Transformative Tool for Antibiotic Resistance Surveillance: Highlighting the Impact of Mobile Genetic Elements with a Focus on the Complex Role of Phages.Antibiotics (Basel, Switzerland) · 2025Review
- A metagenomic perspective on the microbial prokaryotic genome census.Science advances · 2025Article
- Active bacteria driving N2O mitigation and dissimilatory nitrate reduction to ammonium in ammonia recovery bioreactors.The ISME journal · 2025Article
- Physically stable yet biologically sensitive: multiyear ecological dynamics of anoxygenic phototrophs in stably redox-stratified Lake Cadagno.Aquatic sciences · 2025Article
- Uncovering newMicrobiology spectrum · 2024Article
- Biosynthetic potential of the sediment microbial subcommunities of an unexplored karst ecosystem and its ecological implications.MicrobiologyOpen · 2024Article
- Phylogenomic discernments into Anaerolineaceae thermal adaptations and the proposal of a candidate genusFrontiers in microbiology · 2024Article
- Dancing the Nanopore limbo - Nanopore metagenomics from small DNA quantities for bacterial genome reconstruction.BMC genomics · 2023Article
- Metabolic versatility of soil microbial communities below the rocks of the hyperarid Dalangtan Playa.Applied and environmental microbiology · 2023Article
- Trait biases in microbial reference genomes.Scientific data · 2023Article
- Persicamidines-Unprecedented Sesquarterpenoids with Potent Antiviral Bioactivity against Coronaviruses.Angewandte Chemie (International ed. in English) · 2023Article
- Comparative genomic insights into habitat adaptation of coral-associatedFrontiers in microbiology · 2023Article
- Article
- Metagenomic-based surveillance systems for antibiotic resistance in non-clinical settings.Frontiers in microbiology · 2022Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most of Earth's bacteria have yet to be cultivated. The metabolic and functional potentials of these uncultivated microorganisms thus remain mysterious, and the metagenome-assembled genome (MAG) approach is the most robust method for uncovering these potentials. However, MAGs discovered by conventional metagenomic assembly and binning are usually highly fragmented genomes with heterogeneous sequence contamination. In this study, we combined Illumina and Nanopore data to develop a new workflow to reconstruct 233 MAGs-six novel bacterial orders, 20 families, 66 genera, and 154 species-from Lake Shunet, a secluded meromictic lake in Siberia. With our workflow, the average N50 of reconstructed MAGs greatly increased 10-40-fold compared to when the conventional Illumina assembly and binning method were used. More importantly, six complete MAGs were recovered from our datasets. The recovery of 154 novel species MAGs from a rarely explored lake greatly expands the current bacterial genome encyclopedia.
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