ArticleScientific reports2021
Targeted knockout of the gene OsHOL1 removes methyl iodide emissions from rice plants.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Rolling Leaf 2 Controls Leaf Rolling by Regulating Adaxial-Side Bulliform Cell Number and Size in Rice.Plants (Basel, Switzerland) · 2025Article
- Agronomic Biofortification of Plants with Iodine and Selenium: A Potential Solution for Iodine and Selenium Deficiencies.Biological trace element research · 2025Review
- The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation.Plant physiology · 2024Article
- Mainstreaming production and nutrient resilience of vegetable crops in megacities: pre-breeding for terrace cultivation.Frontiers in plant science · 2023Review
- Genome editing.Scientific reports · 2022Article
- Review
- Improvement in fruit yield and tolerance to salinity of tomato plants fertigated with micronutrient amounts of iodine.Scientific reports · 2022Article
- Production of Methyl-Iodide in the Environment.Frontiers in microbiology · 2021Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Iodine deficiency represents a public health problem worldwide. To increase the amount of iodine in the diet, biofortification strategies of plants have been tried. They rely on the exogenous administration of iodine to increase its absorption and accumulation. However, iodine is not stable in plants and can be volatilized as methyl iodide through the action of specific methyltransferases encoded by the HARMLESS TO OZONE LAYER (HOL) genes. The release of methyl iodide in the atmosphere represents a threat for the environment due to its ozone depletion potential. Rice paddies are among the strongest producers of methyl iodide. Thus, the agronomic approach of iodine biofortification is not appropriate for this crop, leading to further increases of iodine emissions. In this work, we used the genome editing CRISPR/Cas9 technology to knockout the rice HOL genes and investigate their function. OsHOL1 resulted a major player in methyl iodide production, since its knockout abolished the process. Moreover, its overexpression reinforced it. Conversely, knockout of OsHOL2 did not produce effects. Our experiments helped elucidating the function of the rice HOL genes, providing tools to develop new rice varieties with reduced iodine emissions and thus more suitable for biofortification programs without further impacting on the environment.
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