ArticleStem cell research & therapy2021
Tissue engineering of mouse uterus using menstrual blood stem cells (MenSCs) and decellularized uterine scaffold.
Article in Stem cell research & therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Bioengineering trends in female reproduction: a systematic review.Human reproduction update · 2022Pooled it
- Candidate Gene Identification: SOX11 Downregulation and IL-7 Upregulation Correlate with the Pro-Proliferative Effect of Mesenchymal Stem Cell-Derived Exosomes on Schwann Cells.Combinatorial chemistry & high throughput screening · 2026Article
- HuMenSCs Initiate the Uterus Stromal Decidualization in Mouse.Current stem cell research & therapy · 2025Article
- Uterine Biosynthesis through Tissue Engineering: An Overview of Current Methods and Status.Current pharmaceutical biotechnology · 2025Review
- Application of biomaterials in mesenchymal stem cell based endometrial reconstruction: current status and challenges.Frontiers in bioengineering and biotechnology · 2025Review
- Acellular porcine placental membranes as a novel biomaterial for tissue repair applications.Frontiers in bioengineering and biotechnology · 2025Article
- Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications.Biomimetics (Basel, Switzerland) · 2024Article
- Advances in Nanomedicine and Biomaterials for Endometrial Regeneration: A Comprehensive Review.International journal of nanomedicine · 2024Review
- Comparative analysis of porcine-uterine decellularization for bioactive-molecule preservation and DNA removal.Frontiers in bioengineering and biotechnology · 2024Article
- Current status and challenges in uterine myometrial tissue engineering.Bioengineered · 2023Review
- The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.International journal of fertility & sterility · 2023Article
- Article
- A Combination of Physical and Chemical Treatments Is More Effective in The Preparation of Acellular Uterine Scaffolds.Cell journal · 2023Article
- Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine.International journal of molecular sciences · 2022Review
- Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies.Frontiers in cell and developmental biology · 2022Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUterine tissue engineering can provide the opportunity for curing female infertility. Natural scaffold is a good choice to recapitulate the architecture and functionality of the native tissue. In this study, we purposed the potential of uterine decellularized scaffolds as an adequate natural niche for MenSCs differentiation toward uterus-specific cell lineages.
methodsMouse's uterus was decellularized by immersion of hypo and hypertonic salts or freeze-thaw cycle followed by immersion in Triton X-100 and SDS solutions. MenSCs were isolated from the menstrual blood of 6 healthy women. The decellularized and recellularized samples were prepared for further in vitro and in vivo analyses.
resultsHistochemical studies and Raman spectroscopy revealed uterine ECM was preserved well, and the cells were completely removed after decellularization. Scanning electron microscopy (SEM) showed that the 3D ultrastructure of the uterus remained intact. Flowcytometric examination with CD34, CD44, CD105, CD106, CD144 markers revealed stem cell characters of cells that isolated from menstrual blood. MTT assay confirmed the bioavailability of MenSCs cultured scaffolds after 7 and 10 days.
conclusionHistochemical studies, SEM images, and Raman spectra showed MenSCs seeded and growth in uterine scaffolds. Immunostaining using anti-cytokeratin (CK), anti-desmin (Des), anti-vimentin (Vim), and anti-smooth muscle actin (SMA) antibodies showed MenSCs differentiation to epithelial and smooth muscle tissues. The Raman spectroscopy revealed the extracellular matrix (ECM) of decellularized uterine scaffolds was well preserved. The decellularized uterus can be considered a promising vehicle to support cell transplantation and differentiation. MenSCs are a good choice for uterine tissue engineering. The complete decellularization from mice uterine tissue was done by combining chemical agents.
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