Evidence map›Paper›PMID 34423284›Full record

ArticleBiosensors & bioelectronics: X2021

A paper-based colorimetric molecular test for SARS-CoV-2 in saliva.

Josiah Levi Davidson, Jiangshan Wang, Murali Kannan Maruthamuthu, Andres Dextre, Ana Pascual-Garrigos, Suraj Mohan, Sai Venkata Sravan Putikam, Fujr Osman Ibrahim Osman, Darby McChesney, Jordan Seville and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics: X, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.

0numbers the graph read from it
0cells of the map it votes in
51citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

51 citing papers in PubMed, 98 citations in OpenAlex.

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  11. Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samples.Proceedings of the National Academy of Sciences of the United States of America · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Josiah Levi DavidsonDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Jiangshan WangDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Murali Kannan MaruthamuthuDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Andres DextreDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Ana Pascual-GarrigosDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Suraj MohanDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Sai Venkata Sravan PutikamDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Fujr Osman Ibrahim OsmanDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Darby McChesneyPortaScience, Inc, Moorestown, NJ, 08057, USA.
Jordan SevillePortaScience, Inc, Moorestown, NJ, 08057, USA.
Mohit S VermaDepartment of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Purdue University West Lafayette · USPortaScience (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/μL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex® backing via an ARclean® double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets.

Indexed as

Colorimetric LAMPMicrofluidic paper-based analytical devicesPaper-based diagnosticsSalivaSARS-CoV-2

Identifiers

PMID34423284
PMCPMC8364207
OpenAlexW3194617893

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.