Evidence map›Paper›PMID 34422774›Full record

ArticleFrontiers in chemistry2021

Designed Artificial Protein Heterodimers With Coupled Functions Constructed Using Bio-Orthogonal Chemistry.

Rachel L Johnson, Hayley G Blaber, Tomas Evans, Harley L Worthy, Jacob R Pope, D Dafydd Jones

Abstract read
In one paragraph

Article in Frontiers in chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Rachel L JohnsonMolecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff, United Kingdom.
Hayley G BlaberMolecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff, United Kingdom.
Tomas EvansMolecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff, United Kingdom.
Harley L WorthyMolecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff, United Kingdom.
Jacob R PopeMolecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff, United Kingdom.
D Dafydd JonesMolecular Biosciences Division, School of Biosciences, Cardiff University, Cardiff, United Kingdom.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The formation of protein complexes is central to biology, with oligomeric proteins more prevalent than monomers. The coupling of functionally and even structurally distinct protein units can lead to new functional properties not accessible by monomeric proteins alone. While such complexes are driven by evolutionally needs in biology, the ability to link normally functionally and structurally disparate proteins can lead to new emergent properties for use in synthetic biology and the nanosciences. Here we demonstrate how two disparate proteins, the haem binding helical bundle protein cytochrome

Indexed as

artificial protein oligomerazide alkyne cycloadditionbioorthogonal chemistryenergy transferfluorescent proteinsgenetic code expansionprotein design and engineering

Identifiers

PMID34422774
PMCPMC8371201

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.