Evidence map›Paper›PMID 34420147›Full record

ArticleMolecular biology reports2021

Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844).

Habibollah Gandomkar, Seyed Pezhman Hosseini Shekarabi, Hossein Ali Abdolhay, Sajad Nazari, Mehdi Shamsaie Mehrgan

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

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5 authors.

Habibollah GandomkarDepartment of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Seyed Pezhman Hosseini ShekarabiDepartment of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran. hosseini.pezhman@yahoo.com.
Hossein Ali AbdolhayAgricultural Research, Education and Extension Organization (AREEO), Iranian Fisheries Sciences Research Institute, Tehran, Iran.
Sajad NazariAgricultural Research, Education and Extension Organization (AREEO), Shahid Motahary Cold-Water Fishes Genetic and Breeding Research Center, Iranian Fisheries Sciences Research Institute, Yasouj, Iran.
Mehdi Shamsaie MehrganDepartment of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran.

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No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemic to Iran. However, the investigation of the population genetic structure of this species is limited by the low number of molecular markers currently described. METHODS AND

resultsIn this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16 (average = 7.89). The results showed that, the polymorphism information content (PIC) of these simple sequence repeat (SSR) loci varied from 0.254 to 0.888. The observed heterozygosity (H

conclusionsThese microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate and other closely related species.

Indexed as

GenotypeAllelesAnimalsBase SequenceCyprinidaeGenetic LociGenetic MarkersGenotyping TechniquesHeterozygoteHigh-Throughput Nucleotide SequencingIranMetagenomicsMicrosatellite RepeatsPolymorphism, GeneticSequence Analysis, DNAGenetic MarkersCapoeta aculeataGenetic structureMicrosatellite markersNext generation sequencing (NGS)

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PMID34420147

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