Evidence map›Paper›PMID 34419745›Full record

ArticleStem cell research2021

Generation of iPSC line from a Parkinson patient with PARK7 mutation and CRISPR-edited Gibco human episomal iPSC line to mimic PARK7 mutation.

Melissa Conti Mazza, Alexandra Beilina, Dorien A Roosen, David Hauser, Mark R Cookson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Melissa Conti MazzaNational Institute on Aging, National Institutes of Health, Bethesda, MD 20892, United States.
Alexandra BeilinaNational Institute on Aging, National Institutes of Health, Bethesda, MD 20892, United States.
Dorien A RoosenNational Institute on Aging, National Institutes of Health, Bethesda, MD 20892, United States.
David HauserNational Institute on Aging, National Institutes of Health, Bethesda, MD 20892, United States.
Mark R CooksonNational Institute on Aging, National Institutes of Health, Bethesda, MD 20892, United States. Electronic address: cookson@mail.nih.gov.
National Institute on Aging · USNational Institutes of Health · US

Funding

DJ-1 function and oxidative stressZ01AG000953 · NIA · NATIONAL INSTITUTE ON AGING · PI COOKSON, MARK R · 2002 to 2008
$2.7M
Intramural NIH HHS Z01 AG000953
6 · The paper itself

Abstract

Mutations in the oncogene PARK7, which codes for DJ-1, have been associated with early-onset autosomal recessive Parkinson's disease (PD); however, the exact role of DJ-1 in PD remains elusive. Fibroblasts from a PD patient with a uniparental disomy, 1 bp deletion in PARK7 were reprogrammed into the induced pluripotent stem cell (iPSC) line: NIHTVBi015-A. For control purposes, CRISPR-Cas9 editing was used to mimic the mutation in the Gibco Human Episomal iPSC line: TMOi001-A is the control line (A18945) and TMOi001-A-3 is the control-edited line (2B10). All 3 lines exhibit normal karyotyping and expression of pluripotent markers: OCT4, SOX2, and NANOG. These lines provide a translational environment to study DJ-1-related function in PD.

Indexed as

Induced Pluripotent Stem CellsParkinson DiseaseFibroblastsHumansMutationProtein Deglycase DJ-1PARK7 protein, humanProtein Deglycase DJ-1

Identifiers

PMID34419745
PMCPMC8451958
OpenAlexW3196280281

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.