ArticleNucleic acids research2021
Formation of artificial chromosomes in Caenorhabditis elegans and analyses of their segregation in mitosis, DNA sequence composition and holocentromere organization.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Targeted integration of extrachromosomal arrays inScience advances · 2026Article
- FLInt 2.0: robust and customizable single-shot integration in C. elegans.G3 (Bethesda, Md.) · 2026Article
- Large transgene arrays cause aberrant transcription and synthetic molting defects withbioRxiv : the preprint server for biology · 2026Article
- FLInt 2.0: Robust and customizable single shot integration inbioRxiv : the preprint server for biology · 2026Article
- High-efficiency targeted integration of extrachromosomal arrays inbioRxiv : the preprint server for biology · 2025Article
- Argonaute protein CSR-1 restricts localization of holocentromere protein HCP-3, the C. elegans CENP-A homolog.Journal of cell science · 2024Article
- The Impact of Chromosomal Rearrangements in Speciation: From Micro- to Macroevolution.Cold Spring Harbor perspectives in biology · 2023Review
- Centromeric and pericentric transcription and transcripts: their intricate relationships, regulation, and functions.Chromosoma · 2023Review
- High-throughput library transgenesis ineLife · 2023Article
- Recurrent but Short-Lived Duplications of Centromeric Proteins in Holocentric Caenorhabditis Species.Molecular biology and evolution · 2022Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Nematode chromosomes.Genetics · 2022Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate how exogenous DNA concatemerizes to form episomal artificial chromosomes (ACs), acquire equal segregation ability and maintain stable holocentromeres, we injected DNA sequences with different features, including sequences that are repetitive or complex, and sequences with different AT-contents, into the gonad of Caenorhabditis elegans to form ACs in embryos, and monitored AC mitotic segregation. We demonstrated that AT-poor sequences (26% AT-content) delayed the acquisition of segregation competency of newly formed ACs. We also co-injected fragmented Saccharomyces cerevisiae genomic DNA, differentially expressed fluorescent markers and ubiquitously expressed selectable marker to construct a less repetitive, more complex AC. We sequenced the whole genome of a strain which propagates this AC through multiple generations, and de novo assembled the AC sequences. We discovered CENP-AHCP-3 domains/peaks are distributed along the AC, as in endogenous chromosomes, suggesting a holocentric architecture. We found that CENP-AHCP-3 binds to the unexpressed marker genes and many fragmented yeast sequences, but is excluded in the yeast extremely high-AT-content centromeric and mitochondrial DNA (> 83% AT-content) on the AC. We identified A-rich motifs in CENP-AHCP-3 domains/peaks on the AC and on endogenous chromosomes, which have some similarity with each other and similarity to some non-germline transcription factor binding sites.
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