ArticlePlant biotechnology journal2021
Glucan, Water-Dikinase 1 (GWD1), an ideal biotechnological target for potential improving yield and quality in rice.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Decoding heat-induced chalkiness in rice: molecular mechanisms, genetic networks, and mitigation strategies for climate resilience.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- QTL Mapping for RVA Profile Characteristics in a Recombinant Inbred Line Population Derived from High-Harvest-Index Yuexiangzhan Rice.Plants (Basel, Switzerland) · 2026Article
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- De novo transcriptome assembly and gene expression analysis of Cnidium officinale under high-temperature conditions.BMC genomics · 2025Article
- Carbohydrate flow during grain filling: Phytohormonal regulation and genetic control in rice (Oryza sativa).Journal of integrative plant biology · 2025Review
- Candidate Gene for Kernel-Related Traits in Maize Revealed by a Combination of GWAS and Meta-QTL Analyses.Plants (Basel, Switzerland) · 2025Article
- Genome-wide identification of starch metabolism gene families in Potentilla anserina and the expression pattern in response to abiotic stress factors.BMC plant biology · 2025Article
- CRISPR mutant rapid identification inFrontiers in plant science · 2025Article
- Article
- Application of CRISPR/Cas9 Technology in Rice Germplasm Innovation and Genetic Improvement.Genes · 2024Review
- Rice Promoter Editing: An Efficient Genetic Improvement Strategy.Rice (New York, N.Y.) · 2024Review
- Starch phosphorylation-A needle in a haystack.Plant methods · 2024Article
- OsLESV and OsESV1 promote transitory and storage starch biosynthesis to determine rice grain quality and yield.Plant communications · 2024Article
- OsCRLK2, a Receptor-Like Kinase Identified by QTL Analysis, is Involved in the Regulation of Rice Quality.Rice (New York, N.Y.) · 2024Article
- RGA1 regulates grain size, rice quality and seed germination in the small and round grain mutant srg5.BMC plant biology · 2024Article
- Salinity inhibits seed germination and embryo growth by reducing starch mobilization efficiency in barley.Plant direct · 2024Article
- Transcriptomics and starch biosynthesis analysis in leaves and developing seeds of mung bean provide a basis for genetic engineering of starch composition and seed quality.Frontiers in plant science · 2024Article
- Peculiar properties of tuber starch in a potato mutant lacking the α-glucan water dikinase 1 genePlant biotechnology (Tokyo, Japan) · 2023Article
- A syntelog-based pan-genome provides insights into rice domestication and de-domestication.Genome biology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The source-sink relationship determines the overall agronomic performance of rice. Cloning and characterizing key genes involved in the regulation of source and sink dynamics is imperative for improving rice yield. However, few source genes with potential application in rice have been identified. Glucan, Water-Dikinase 1 (GWD1) is an essential enzyme that plays a pivotal role in the first step of transitory starch degradation in source tissues. In the present study, we successfully generated gwd1 weak mutants by promoter editing using CRISPR/Cas9 system, and also leaf-dominant overexpression lines of GWD1 driven by Osl2 promoter. Analysis of the gwd1 plants indicated that promoter editing mediated down-regulation of GWD1 caused no observable effects on rice growth and development, but only mildly modified its grain transparency and seed germination. However, the transgenic pOsl2::GWD1 overexpression lines showed improvements in multiple key traits, including rice yield, grain shape, rice quality, seed germination and stress tolerance. Therefore, our study shows that GWD1 is not only involved in transitory starch degradation in source tissues, but also plays key roles in the seeds, which is a sink tissue. In conclusion, we find that GWD1 is an ideal biotechnological target with promising potential for the breeding of elite rice cultivars via genetic engineering.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.