ArticleMetallomics : integrated biometal science2021
A genome-wide screen reveals the involvement of enterobactin-mediated iron acquisition in Escherichia coli survival during copper stress.
Article in Metallomics : integrated biometal science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 17 citations in OpenAlex.
- Long-chain fatty acid homeostasis contributes to survival of uropathogenicJournal of bacteriology · 2026Article
- Copper stress upregulates oxidative stress response, histidine production, and iron acquisition genes inFEMS microbes · 2026Article
- Antibacterial activity of green-synthesized silver nanoparticles against Gram-negative bacteria and insights into potential resistance mechanisms.INNOSC theranostics & pharmacological sciences · 2025Article
- Role of a TonB-dependent receptor and an oxygenase in iron-dependent copper resistance inJournal of bacteriology · 2025Article
- Fecal carriage and molecular characterization of carbapenem-resistantFrontiers in microbiology · 2025Article
- Identification of a copper-responsive small molecule inhibitor of uropathogenicJournal of bacteriology · 2024Article
- The inner membrane protein YhiM links copper and CpxAR envelope stress responses in uropathogenicmBio · 2024Article
- Genome mining of Escherichia coli WG5D from drinking water source: unraveling antibiotic resistance genes, virulence factors, and pathogenicity.BMC genomics · 2024Article
- Can antimicrobial blue light contribute to resistance development? Genome-wide analysis revealed aBL-protective genes inMicrobiology spectrum · 2024Article
- Mis-regulation of Zn and Mn homeostasis is a key phenotype of Cu stress in Streptococcus pyogenes.Metallomics : integrated biometal science · 2023Article
- Adaptive Mechanisms ofToxics · 2023Article
- Article
- Gradients in gene essentiality reshape antibacterial research.FEMS microbiology reviews · 2022Review
- Less is more: Enterobactin concentration dependency in copper tolerance and toxicity.Frontiers in molecular biosciences · 2022Article
- Antibiotic resistance and host immune system-induced metal bactericidal control are key factors for microbial persistence in the developing human preterm infant gut microbiome.Frontiers in microbiology · 2022Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Copper (Cu) is a key transition metal that is involved in many important biological processes in a cell. Cu is also utilized by the immune system to hamper pathogen growth during infection. However, genome-level knowledge on the mechanisms involved in adaptation to Cu stress is limited. Here, we report the results of a genome-wide reverse genetic screen for Cu-responsive phenotypes in Escherichia coli. Our screen has identified novel genes involved in adaptation to Cu stress in E. coli. We detected multiple genes involved in the biosynthesis and uptake of enterobactin, a siderophore utilized for high-affinity TonB-dependent acquisition of iron (Fe), as critical players in survival under Cu intoxication. We demonstrated the specificity of Cu-dependent killing by chelation of Cu and by genetic complementation of tonB. Notably, TonB is involved in protection from Cu in both laboratory and uropathogenic strains of E. coli. Cu stress leads to increased expression of the genes involved in Fe uptake, indicating that Fur regulon is derepressed during exposure to excess Cu. Trace element analyses revealed that Fe homeostasis is dysregulated during Cu stress. Taken together, our data supports a model in which lack of enterobactin-dependent Fe uptake leads to exacerbation of Cu toxicity, and elucidates the intricate connection between the homeostasis of Cu and Fe in a bacterial cell.
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