ArticleThe journal of applied laboratory medicine2021
Sample Processing and Stability for Urine Biomarker Studies.
Article in The journal of applied laboratory medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Urinary placental growth factor stability as a critical factor in the reliability of preeclampsia diagnosis.Biochemia medica · 2026Article
- Operational checks for real-time urinary biomarker measurements for assessment of the quality of deceased donor kidneys.Scientific reports · 2026Article
- Multimodal neuroimmune diagnostic profiling for pediatric lower urinary tract dysfunction associated with occult sacral nerve abnormalities.Frontiers in immunology · 2026Article
- Novel Kidney Biomarkers and Antibiotics-Induced Acute Kidney Injury: A Practical Assessment of Current and Future Applications.Clinical and translational science · 2025Review
- Development and Validation of Multiplex Assays for Lupus Nephritis Activity Biomarkers.Kidney international reports · 2025Article
- Effects of Different Voided Urine Sample Storage Time, Temperature, and Preservatives on Analysis with Multiplex Bead-Based Oncuria Bladder Cancer Immunoassay.Diagnostics (Basel, Switzerland) · 2025Article
- Reference values of urinary metabolites of organophosphate in healthy Iranian adults.PloS one · 2025Article
- Immunomonitoring in bladder cancer: a short report on stability of leukocytes and proteins in refrigerated urine samples.BMC urology · 2024Article
- Analytical Insights into Methods for Measuring Ischemia-Modified Albumin.Molecules (Basel, Switzerland) · 2024Review
- Advancements in Trauma-Induced Acute Kidney Injury: Diagnostic and Therapeutic Innovations.Life (Basel, Switzerland) · 2024Review
- PHENOTYPING REPAIR AFTER ACUTE KIDNEY INJURY: PRECISION MEDICINE TO CLINICAL TRIALS.Transactions of the American Clinical and Climatological Association · 2024Article
- Investigation of select radionuclides stability in urine under various conditions for liquid scintillation counting (LSC).Journal of radioanalytical and nuclear chemistry · 2024Article
- Evaluation of Plasma Biomarkers to Predict Major Adverse Kidney Events in Hospitalized Patients With COVID-19.American journal of kidney diseases : the official journal of the National Kidney Foundation · 2023Article
- Acute kidney injury biomarkers and hydration assessments following prolonged mild hypohydration in healthy young adults.American journal of physiology. Renal physiology · 2023Article
- Stability of novel urinary biomarkers used for lupus nephritis.Frontiers in pediatrics · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCurrent methods of processing and storing urine samples have not been compared systematically to determine optimal conditions for advancing research on urinary biomarkers. Often, preanalytical handling is nonideal, especially considering the COVID-19 pandemic; consequently, we compared the effects of different short-term storage and processing methods on urinary biomarker measurements.
methodsSpot urine samples were collected via a Foley catheter from 20 hospitalized patients from the Yale New Haven Hospital within 48 hours postcardiac surgery. The effects of 3 urine storage and processing methods on biomarkers were tested: (a) 48-hour temporary storage at 4 °C prior to freezing at -80 °C, (b) 48-hour temporary storage at 25 °C prior to freezing at -80 °C, and (c) no centrifugation and immediate storage at -80 °C. Established Meso-Scale Device assay methods were used to measure the urine concentrations of 18 biomarkers: interferon gamma (IFN-ɣ), interleukin (IL)-10, IL-12p70, IL-13, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-18, tumor necrosis factor alpha (TNF-α), epidermal growth factor (EGF), neutrophil gelatinase-associated lipocalin (NGAL), osteopontin (OPN), uromodulin (UMOD), kidney injury molecule-1 (KIM-1), monocyte chemoattractant protein-1 (MCP-1), and chitinase-3-like protein 1 (YKL-40).
resultsMeasurements of most biomarkers investigated remained stable after temporary storage at 4 °C. IL-6, IL-8, KIM-1, MCP-1, YKL-40, EGF, and NGAL were stable across all 3 processing conditions. IL-12p70 and IL-4 demonstrated significant differences in all tested conditions compared to the reference standard.
conclusionsWe identified several notable biomarkers that are robust to variations in preanalytical techniques and can be reliably investigated with nonideal handling conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.