ArticleScientific reports2021
Identifying volatile in vitro biomarkers for oral bacteria with proton-transfer-reaction mass spectrometry and gas chromatography-mass spectrometry.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Ectopic Olfactory Receptors in Oral Health and Disease: Molecular Links Between Chemosensing, Tissue Repair, Inflammation, and Cancer.International journal of molecular sciences · 2026Review
- VOC Emission Screening of Consumer Products in Microchambers: Comparison of Online PTR-MS and Offline TD-GC-MS Analysis.Analytical chemistry · 2026Article
- Volatilomic complexity of three Northern Greenland bacterial isolates across a salt gradient.FEMS microbiology ecology · 2025Article
- Studies on the Emission of Volatile Organic Compounds from Selected Forest Mushrooms of the GenusMolecules (Basel, Switzerland) · 2025Article
- Bacterial Volatile Organic Compounds as Potential Caries and Periodontitis Disease Biomarkers.International journal of molecular sciences · 2025Article
- Relationship Between the Salivary Microbiome and Oral Malodor Metabolites in Older Thai Individuals with Periodontitis and the Cytotoxic Effects of Malodor Compounds on Human Oral Squamous Carcinoma (HSC-4) Cells.Dentistry journal · 2025Article
- Periodontitis biomarkers through thermal desorption-gas chromatography-mass spectrometry analysis.Frontiers in dental medicine · 2025Article
- Rapid Identification of Carbapenem-ResistantInfection and drug resistance · 2025Article
- Combination of real-time and hyphenated mass spectrometry for improved characterisation of exhaled breath biomarkers in clinical research.Analytical and bioanalytical chemistry · 2024Article
- Oral microbiota and metabolites: key players in oral health and disorder, and microbiota-based therapies.Frontiers in microbiology · 2024Review
- A State of Art Review: Volatile Organic Compounds and Periodontitis.Galen medical journal · 2024Review
- Diagnosis by Volatile Organic Compounds in Exhaled Breath in Exhaled Breath from Patients with Gastric and Colorectal Cancers.International journal of molecular sciences · 2022Review
- Diagnosis and prognosis of COVID-19 employing analysis of patients' plasma and serum via LC-MS and machine learning.Computers in biology and medicine · 2022Article
- Thiol Metabolism and Volatile Metabolome ofFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have measured the volatile fingerprints of four pathogenic oral bacteria connected to periodontal disease and dental abscess: Porphyromonas gingivalis (three separate strains), Prevotella intermedia, Prevotella nigrescens and Tannerella forsythia. Volatile fingerprints were measured in vitro from the headspace gas of the bacteria cultured on agar. Concrete identification of new and previously reported bacterial volatiles were performed by a combination of solid phase microextraction (SPME) and offline gas chromatography-mass spectrometry (GC-MS). We also studied the effect of the reduced electric field strength (E/N) on the fragmentation patterns of bacterial volatiles in online proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). We aimed to discover possible new biomarkers for the studied oral bacteria, as well as to validate the combination of GC-MS and PTR-MS for volatile analysis. Some of the most promising compounds produced include: 1-Methyl-1,2,3,4-tetrahydroisoquinoline (1MeTIQ), indole, and a cascade of sulphur compounds, such as methanethiol, dimethyl disulphide (DMDS) and dimethyl trisulphide (DMTS). We also found that several compounds, especially alcohols, aldehydes and esters, fragment significantly with the PTR-MS method, when high E/N values are used. We conclude that the studied oral bacteria can be separated by their volatile fingerprints in vitro, which could have importance in clinical and laboratory environments. In addition, using softer ionization conditions can improve the performance of the PTR-MS method in the volatile analysis of certain compounds.
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