Evidence map›Paper›PMID 34413232›Full record

ArticleScience (New York, N.Y.)2021

Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery.

Michael Segel, Blake Lash, Jingwei Song, Alim Ladha, Catherine C Liu, Xin Jin, Sergei L Mekhedov, Rhiannon K Macrae, Eugene V Koonin, Feng Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 246 papers.

0numbers the graph read from it
0cells of the map it votes in
246citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

246 citing papers in PubMed.

  1. In vivo CRISPR gene editing in patients with herpetic stromal keratitis.Molecular therapy : the journal of the American Society of Gene Therapy · 2023
    Trial
  2. Overview of Delivery Methods for Gene Editing.Methods in molecular biology (Clifton, N.J.) · 2027
    Review
  3. Review
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  5. Article
  6. Review
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  8. Article
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  14. Acta pharmaceutica Sinica. B · 2026
    Review
  15. Review
  16. Article
  17. Article
  18. Article
  19. In Vivo T-Cell Engineering: Revolution in Delivery Strategies and Clinical Translation.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026
    Review
  20. Article

186 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Michael SegelHoward Hughes Medical Institute, Cambridge, MA 02139, USA.ORCID 0000-0001-8986-7417
Blake LashHoward Hughes Medical Institute, Cambridge, MA 02139, USA.ORCID 0000-0003-2445-7948
Jingwei SongHoward Hughes Medical Institute, Cambridge, MA 02139, USA.ORCID 0000-0002-4246-8686
Alim LadhaHoward Hughes Medical Institute, Cambridge, MA 02139, USA.ORCID 0000-0003-3945-7924
Catherine C LiuDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.ORCID 0000-0002-2093-7170
Xin JinBroad Institute of MIT and Harvard, Cambridge, MA 02142, USA.ORCID 0000-0003-2344-992X
Sergei L MekhedovNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
Rhiannon K MacraeHoward Hughes Medical Institute, Cambridge, MA 02139, USA.ORCID 0000-0002-7850-9056
Eugene V KooninNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.ORCID 0000-0003-3943-8299
Feng ZhangHoward Hughes Medical Institute, Cambridge, MA 02139, USA.ORCID 0000-0003-0178-7995

Funding

Finding Protein Sequence Motifs--Methods And ApplicationsZIALM000061 · NLM · NATIONAL LIBRARY OF MEDICINE · PI KOONIN, EUGENE V · 2009 to 2025
$10.6M
Programmable RNA-targeting toolsR01HG009761 · NHGRI · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2017 to 2024
$7.5M
Exploration of Diverse Mobile Genetic Elements for Precision Genome ManipulationDP1HL141201 · NHLBI · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2017 to 2021
$6.2M
Howard Hughes Medical InstituteNHGRI NIH HHS R01 HG009761NHLBI NIH HHS DP1 HL141201
6 · The paper itself

Abstract

Eukaryotic genomes contain domesticated genes from integrating viruses and mobile genetic elements. Among these are homologs of the capsid protein (known as Gag) of long terminal repeat (LTR) retrotransposons and retroviruses. We identified several mammalian Gag homologs that form virus-like particles and one LTR retrotransposon homolog, PEG10, that preferentially binds and facilitates vesicular secretion of its own messenger RNA (mRNA). We showed that the mRNA cargo of PEG10 can be reprogrammed by flanking genes of interest with

Indexed as

AnimalsApoptosis Regulatory ProteinsCapsidCell LineDNA-Binding ProteinsExtracellular VesiclesGene EditingGenetic VectorsHumansMiceNeuronsProtein DomainsRetroelementsRNA-Binding ProteinsRNA, MessengerTransfectionApoptosis Regulatory ProteinsDNA-Binding ProteinsPEG10 protein, humanPeg10 protein, mouseRetroelementsRNA-Binding ProteinsRNA, MessengerUntranslated Regions

Identifiers

PMID34413232
PMCPMC8431961

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.