ArticleJournal of environmental sciences (China)2021
Comparison of approaches to quantify SARS-CoV-2 in wastewater using RT-qPCR: Results and implications from a collaborative inter-laboratory study in Canada.
Article in Journal of environmental sciences (China), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Implementation and Early Outcomes of Laboratory Proficiency Testing Program for the National Wastewater Surveillance System, United States, 2024.Emerging infectious diseases · 2026Article
- Solid Evidence and Liquid Gold: Trade-Offs of Processing Settled Solids, Whole Influent, or Centrifuged Influent for Codetecting Viral, Bacterial, and Eukaryotic Pathogens in Wastewater.Environmental science & technology · 2025Article
- Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study.Frontiers in microbiology · 2025Article
- The effects of RT-qPCR standards on reproducibility and comparability in monitoring SARS-CoV-2 levels in wastewater.Scientific reports · 2024Article
- Optimizing Wastewater Surveillance: The Necessity of Standardized Reporting and Proficiency for Public Health.American journal of public health · 2024Article
- Inter-institutional laboratory standardization for SARS-CoV-2 surveillance through wastewater-based epidemiology applied to Mexico City.IJID regions · 2024Article
- SARS-CoV-2 viral titer measurements in Ontario, Canada wastewaters throughout the COVID-19 pandemic.Scientific data · 2024Article
- Analytical validation of a semi-automated methodology for quantitative measurement of SARS-CoV-2 RNA in wastewater collected in northern New England.Microbiology spectrum · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Quantification and Differentiation of SARS-CoV-2 Variants in Wastewater for Surveillance.Environment & health (Washington, D.C.) · 2023Article
- Adsorption of Respiratory Syncytial Virus, Rhinovirus, SARS-CoV-2, and F+ Bacteriophage MS2 RNA onto Wastewater Solids from Raw Wastewater.Environmental science & technology · 2023Article
- An efficient method to enhance recovery and detection of SARS-CoV-2 RNA in wastewater.Journal of environmental sciences (China) · 2023Article
- Influence of membrane pore-size on the recovery of endogenous viruses from wastewater using an adsorption-extraction method.Journal of virological methods · 2023Article
- Article
- Evaluation of intra- and inter-lab variability in quantifying SARS-CoV-2 in a state-wide wastewater monitoring network.Environmental science : water research & technology · 2023Article
- Number of COVID-19 cases required in a population to detect SARS-CoV-2 RNA in wastewater in the province of Alberta, Canada: Sensitivity assessment.Journal of environmental sciences (China) · 2023Article
- Sensitivity of wastewater surveillance: What is the minimum COVID-19 cases required in population for SARS-CoV-2 RNA to be detected in wastewater?Journal of environmental sciences (China) · 2023Article
- Comparative analysis of Adsorption-Extraction (AE) and Nanotrap® Magnetic Virus Particles (NMVP) workflows for the recovery of endogenous enveloped and non-enveloped viruses in wastewater.The Science of the total environment · 2023Article
- Persistence of endogenous RNA biomarkers of SARS-CoV-2 and PMMoV in raw wastewater: Impact of temperature and implications for wastewater-based epidemiology.The Science of the total environment · 2023Article
- COVID-19 and Water Variables: Review and Scientometric Analysis.International journal of environmental research and public health · 2023Review
Corrections and comments
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Authors and funding
20 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of SARS-CoV-2 RNA in wastewater is a promising tool for informing public health decisions during the COVID-19 pandemic. However, approaches for its analysis by use of reverse transcription quantitative polymerase chain reaction (RT-qPCR) are still far from standardized globally. To characterize inter- and intra-laboratory variability among results when using various methods deployed across Canada, aliquots from a real wastewater sample were spiked with surrogates of SARS-CoV-2 (gamma-radiation inactivated SARS-CoV-2 and human coronavirus strain 229E [HCoV-229E]) at low and high levels then provided "blind" to eight laboratories. Concentration estimates reported by individual laboratories were consistently within a 1.0-log
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Registered trials
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