ArticleToxicology2021
Reproducibility of adipogenic responses to metabolism disrupting chemicals in the 3T3-L1 pre-adipocyte model system: An interlaboratory study.
Article in Toxicology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Maternal exposure to biosolids reshapes chemical burden and steroidogenic pathways to promote adipogenic differentiation in a sheep model.Environment international · 2026Article
- In Vitro Model of Cytokine-Induced Inflammatory 3T3-L1 Adipocytes Mimicking Obesity.Bio-protocol · 2026Article
- Liquid Crystal Monomers and Their Mixtures Alter Nuclear Receptor Signaling and Promote Adipogenesis In Vitro.Endocrinology · 2025Article
- Metabolism disrupting effects in human pre-adipocytes: Influence of donor sex and body composition.Toxicology and applied pharmacology · 2025Article
- Delineating lipidomic landscapes in human and mouse ovaries: Spatial signatures and chemically-induced alterations via MALDI mass spectrometry imaging: Spatial ovarian lipidomics.Environment international · 2024Article
- The herbicide acetochlor causes lipid peroxidation by inhibition of glutathione peroxidase activity.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Nonylphenol and Cetyl Alcohol Polyethoxylates Disrupt Thyroid Hormone Receptor Signaling to Disrupt Metabolic Health.Endocrinology · 2024Article
- Molecular Assessment of Proadipogenic Effects for Common-Use Contraceptives and Their Mixtures.Endocrinology · 2024Article
- Nasopharyngeal Carcinoma Cell Lines: Reliable Alternatives to Primary Nasopharyngeal Cells?Cells · 2024Article
- Addressing chemically-induced obesogenic metabolic disruption: selection of chemicals forFrontiers in endocrinology · 2024Review
- Adipogenic and endocrine disrupting mixture effects of organic and inorganic pollutant mixtures.The Science of the total environment · 2023Article
- Organotin mixtures reveal interactions that modulate adipogenic differentiation in 3T3-L1 preadipocytes.Archives of toxicology · 2023Article
- Triazole fungicides induce adipogenesis and repress osteoblastogenesis in zebrafish.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Cetyl Alcohol Polyethoxylates Disrupt Metabolic Health in Developmentally Exposed Zebrafish.Metabolites · 2023Article
- Development of new approach methods for the identification and characterization of endocrine metabolic disruptors-a PARC project.Frontiers in toxicology · 2023Article
- Article
- Obesity II: Establishing causal links between chemical exposures and obesity.Biochemical pharmacology · 2022Review
- Obesity III: Obesogen assays: Limitations, strengths, and new directions.Biochemical pharmacology · 2022Review
- Nonylphenol Polyethoxylates Enhance Adipose Deposition in Developmentally Exposed Zebrafish.Toxics · 2022Article
- Exposure to Low Doses of Oxybenzone During Perinatal Development Alters Mammary Gland Stroma in Female Mice.Frontiers in toxicology · 2022Article
Corrections and comments
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Authors and funding
23 authors at 12 institutions in 7 countries.
Funding
Abstract
The 3T3-L1 murine pre-adipocyte line is an established cell culture model for screening Metabolism Disrupting Chemicals (MDCs). Despite a need to accurately identify MDCs for further evaluation, relatively little research has been performed to comprehensively evaluate reproducibility across laboratories, assess factors that might contribute to varying degrees of differentiation between laboratories (media additives, plastics, cell source, etc.), or to standardize protocols. As such, the goals of this study were to assess interlaboratory variability of efficacy and potency outcomes for triglyceride accumulation and pre-adipocyte proliferation using the mouse 3T3-L1 pre-adipocyte cell assay to test chemicals. Ten laboratories from five different countries participated. Each laboratory evaluated one reference chemical (rosiglitazone) and three blinded test chemicals (tributyltin chloride, pyraclostrobin, and bisphenol A) using: 1) their Laboratory-specific 3T3-L1 Cells (LC) and their Laboratory-specific differentiation Protocol (LP), 2) Shared 3T3-L1 Cells (SC) with LP, 3) LC with a Shared differentiation Protocol (SP), and 4) SC with SP. Blinded test chemical responses were analyzed by the coordinating laboratory. The magnitude and range of bioactivities reported varied considerably across laboratories and test conditions, though the presence or absence of activity for each tested chemical was more consistent. Triglyceride accumulation activity determinations for rosiglitazone ranged from 90 to 100% across test conditions, but 30-70 % for pre-adipocyte proliferation; this was 40-80 % for triglyceride accumulation induced by pyraclostrobin, 80-100 % for tributyltin, and 80-100 % for bisphenol A. Consistency was much lower for pre-adipocyte proliferation, with 30-70 % active determinations for pyraclostrobin, 30-50 % for tributyltin, and 20-40 % for bisphenol A. Greater consistency was observed for the SC/SP assessment. As such, working to develop a standardized adipogenic differentiation protocol represents the best strategy for improving consistency of adipogenic responses using the 3T3-L1 model to reproducibly identify MDCs and increase confidence in reported outcomes.
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