ArticleJournal of insect physiology2021
Microbiome reduction prevents lipid accumulation during early diapause in the northern house mosquito, Culex pipiens pipiens.
Article in Journal of insect physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Crosstalk in the cold: host-microbe interactions in insect diapause.Annals of the Entomological Society of America · 2026Review
- Characterizing the Microbiome and Prevalence of Wolbachia in Culex pipiens Complex and Culex restuans Mosquitoes in the Midwest United States.Microbial ecology · 2026Article
- Defensive symbionts of the European beewolf face competition from brood cell microbiota during vertical transmission.FEMS microbiology ecology · 2026Article
- Lipid Metabolism in Diapause.Advances in experimental medicine and biology · 2026Review
- Insect Lipid Metabolism in the Presence of Symbiotic and Pathogenic Viruses and Bacteria.Advances in experimental medicine and biology · 2026Review
- Temperature regulation mechanisms of diapause inFrontiers in microbiology · 2026Article
- Transcriptional Control of Lipid Metabolism.Advances in experimental medicine and biology · 2026Review
- Differences among populations and changes during domestication in the lipidome of Queensland fruit fly.BMC ecology and evolution · 2025Article
- Correlation of Midgut Microbiota and Metabolic Syndrome-Related Lipids in Hemolymph Between Obese and Lean Silkworm Strains.Insects · 2025Article
- Microbiota as Potential Functional Traits Facilitating Springtail Activity in Winter.Ecology and evolution · 2025Article
- The Potential Implications of Sex-Specific Differences in the Intestinal Bacteria of the Overwintering Wolf SpiderInsects · 2024Article
- Genome-wide profiles of H3K9me3, H3K27me3 modifications, and DNA methylation during diapause of Asian corn borer (Genome research · 2024Article
- Symbiotic bacteria and fungi proliferate in diapause and may enhance overwintering survival in a solitary bee.The ISME journal · 2024Article
- Microbiota in disease-transmitting vectors.Nature reviews. Microbiology · 2023Review
- A Culex quinquefasciatus strain resistant to the binary toxin from Lysinibacillus sphaericus displays altered enzyme activities and energy reserves.Parasites & vectors · 2023Article
- Lipid metabolism dysfunction following symbiont elimination is linked to altered Kennedy pathway homeostasis.iScience · 2023Article
- Many parasitoids lack adult fat accumulation, despite fatty acid synthesis: A discussion of concepts and considerations for future research.Current research in insect science · 2023Review
- Reshuffling of the Coral Microbiome during Dormancy.Applied and environmental microbiology · 2022Article
- Metabolite Changes in Orange Dead Leaf ButterflyMetabolites · 2022Article
- Bacterial Communities of Lab and Field Northern House Mosquitoes (Diptera: Culicidae) Throughout Diapause.Journal of medical entomology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The mosquito microbiome is critical to multiple facets of their biology, including larval development and disease transmission. For mosquitoes that reside in temperate regions, periods of diapause are critical to overwintering survival, but how the microbiome impacts this state is unknown. In this study, we compared the midgut microbial communities of diapausing and non-diapausing Culex pipiens and assessed how a reduced midgut microbiome influences diapause preparation. High community variability was found within and between non-diapausing and diapausing individuals, but no specific diapause-based microbiome was noted. Emergence of adult, diapausing mosquitoes under sterile conditions generated low bacterial load (LBL) lines with nearly a 1000-fold reduction in bacteria levels. This reduction in bacterial content resulted in significantly lower survival of diapausing females after two weeks, indicating acquisition of the microbiome in adult females is critical for survival throughout diapause. LBL diapausing females had high carbohydrate levels, but did not accumulate lipid reserves, suggesting an inability to process ingested sugars necessary for diapause-associated lipid accumulation. Expression patterns of select genes associated with mosquito lipid metabolism during diapause showed no significant differences between LBL and control lines, suggesting transcriptional changes may not underlie impaired lipid accumulation. Overall, a diverse, adult-acquired microbiome is critical for diapause in C. pipiens to process sugar reserves and accumulate lipids that are necessary to survive prolonged overwintering.
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