ArticleFrontiers in cellular neuroscience2021
Passive Clearing and 3D Lightsheet Imaging of the Intact and Injured Spinal Cord in Mice.
Article in Frontiers in cellular neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- CX3CR1 modulates acute disc herniation-induced pain via regulating local inflammation and spinal microglia activation.Osteoarthritis and cartilage · 2025Article
- Recombinant human DNase treatment mitigates extracellular trap mediated damage and improves long-term recovery after spinal cord injury in male mice.Brain, behavior, and immunity · 2025Article
- Morphological segmentation with tiling light sheet microscopy to quantitatively analyze the three-dimensional structures of spinal motoneurons.Cell regeneration (London, England) · 2025Article
- Progress on three-dimensional visualizing skin architecture with multiple immunofluorescence staining and tissue-clearing approaches.Histology and histopathology · 2025Review
- Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice.Frontiers in neuroscience · 2025Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- A single-cell transcriptomic map of the developing Atoh1 lineage identifies neural fate decisions and neuronal diversity in the hindbrain.Developmental cell · 2024Article
- V2a neurons restore diaphragm function in mice following spinal cord injury.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Foxi3 transcription factor is necessary for the fate restriction of placodal lineages at the neural plate border.Development (Cambridge, England) · 2023Article
- Tissue optical clearing for neural regeneration research.Neural regeneration research · 2023Article
- CRISPR-clear imaging of melanin-rich B16-derived solid tumors.Communications biology · 2023Article
- Article
- Navigating the Light-Sheet Image Analysis Software Landscape: Concepts for Driving Cohesion From Data Acquisition to Analysis.Frontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 1 country.
Funding
Abstract
The spinal cord contains a diverse array of sensory and motor circuits that are essential for normal function. Spinal cord injury (SCI) permanently disrupts neural circuits through initial mechanical damage, as well as a cascade of secondary injury events that further expand the spinal cord lesion, resulting in permanent paralysis. Tissue clearing and 3D imaging have recently emerged as promising techniques to improve our understanding of the complex neural circuitry of the spinal cord and the changes that result from damage due to SCI. However, the application of this technology for studying the intact and injured spinal cord remains limited. Here, we optimized the passive CLARITY technique (PACT) to obtain gentle and efficient clearing of the murine spinal cord without the need for specialized equipment. We demonstrate that PACT clearing enables 3D imaging of multiple fluorescent labels in the spinal cord to assess molecularly defined neuronal populations, acute inflammation, long-term tissue damage, and cell transplantation. Collectively, these procedures provide a framework for expanding the utility of tissue clearing to enhance the study of spinal cord neural circuits, as well as cellular- and tissue-level changes that occur following SCI.
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Registered trials
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