ArticleJournal of exposure science & environmental epidemiology2022
The size and culturability of patient-generated SARS-CoV-2 aerosol.
Article in Journal of exposure science & environmental epidemiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
75 citing papers in PubMed, 2 syntheses or guidelines pooled it, 144 citations in OpenAlex.
- Viral cultures for assessing airborne infectiousness of SARS-CoV-2: a systematic review and meta-analysis.BMC infectious diseases · 2025Pooled it
- SARS-CoV-2 air sampling: A systematic review on the methodologies for detection and infectivity.Indoor air · 2022Pooled it
- Portable air cleaners and residential exposure to SARS-CoV-2 aerosols: A real-world study.Indoor air · 2022Trial
- Climatic Influence on COVID-19: Investigating the Role of Temperature and Humidity in the Spread of the Omicron Variant.GeoHealth · 2026Article
- Understanding Aerosol-Mediated Disease Transmission.ACS central science · 2025Review
- A Microfluidic Chip-Based Integrated Device Combining Aerosol Sampling and LAMP-CRISPR Detection for Airborne Virus Surveillance.Biosensors · 2025Article
- Detection of SARS-CoV-2 in bioaerosols and surface samples from healthcare facilities in Klang Valley, Malaysia.Scientific reports · 2025Article
- Measuring the fitted filtration efficiency of cloth masks, medical masks and respirators.PloS one · 2025Article
- Efficacy of powered air-purifying respirators (PAPRs) for source control of simulated respiratory aerosols.American journal of infection control · 2024Article
- LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages.Nature communications · 2024Article
- Multi-scenario surveillance of respiratory viruses in aerosols with sub-single-copy spatial resolution.Nature communications · 2024Article
- SARS-CoV-2 infection and transmission via the skin to oro-nasal route with the production of bioaerosols in the ferret model.The Journal of general virology · 2024Article
- On modelling airborne infection risk.Royal Society open science · 2024Article
- Estimated relative potential for airborne SARS-CoV-2 transmission in a day care centre.Heliyon · 2024Article
- Frequency, kinetics and determinants of viable SARS-CoV-2 in bioaerosols from ambulatory COVID-19 patients infected with the Beta, Delta or Omicron variants.Nature communications · 2024Article
- Air Change Rate and SARS-CoV-2 Exposure in Hospitals and Residences: A Meta-Analysis.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2024Article
- The BioCascade-VIVAS system for collection and delivery of virus-laden size-fractionated airborne particles.Journal of aerosol science · 2024Article
- Association between in situ ventilation and human-generated aerosol exposure in meatpacking plants during the COVID-19 pandemic.PloS one · 2024Article
- On-site airborne pathogen detection for infection risk mitigation.Chemical Society reviews · 2023Review
- Airborne SARS-CoV-2 is more frequently detected in environments related to children and elderly but likely non-infectious, Norway, 2022.Virology journal · 2023Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAerosol transmission of COVID-19 is the subject of ongoing policy debate. Characterizing aerosol produced by people with COVID-19 is critical to understanding the role of aerosols in transmission.
objectiveWe investigated the presence of virus in size-fractioned aerosols from six COVID-19 patients admitted into mixed acuity wards in April of 2020.
methodsSize-fractionated aerosol samples and aerosol size distributions were collected from COVID-19 positive patients. Aerosol samples were analyzed for viral RNA, positive samples were cultured in Vero E6 cells. Serial RT-PCR of cells indicated samples where viral replication was likely occurring. Viral presence was also investigated by western blot and transmission electron microscopy (TEM).
resultsSARS-CoV-2 RNA was detected by rRT-PCR in all samples. Three samples confidently indicated the presence of viral replication, all of which were from collected sub-micron aerosol. Western blot indicated the presence of viral proteins in all but one of these samples, and intact virions were observed by TEM in one sample. SIGNIFICANCE: Observations of viral replication in the culture of submicron aerosol samples provides additional evidence that airborne transmission of COVID-19 is possible. These results support the use of efficient respiratory protection in both healthcare and by the public to limit transmission.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.