ArticleScientific reports2021
Calcium silicate-based cements cause environmental stiffness and show diverse potential to induce osteogenesis in human osteoblastic cells.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- In Vitro Biocompatibility of Calcium Silicate-Based Materials for Retrograde Endodontic Treatment Under Different Setting Conditions.Journal of functional biomaterials · 2026Article
- Evaluation of marginal adaptation and bond strength of apical root canal plugs using different bioceramic cements.BMC oral health · 2026Article
- In vitro and in vivo biocompatibility and bioactivity of bioceramic repair materials.Brazilian oral research · 2026Article
- Histological and radiographic assessment of the regenerative potential of sodium hexametaphosphate (SHMP) as a novel direct pulp capping material in an animal model.BMC oral health · 2025Article
- Nonsurgical Retreatment of a 16 × 9 mm Osteolytic Lesion With Buccal Bone Regeneration Using a Bioceramic Sealer: A Clinical and NGS-Based Microbiological Case Report With 30-Month Follow-Up.Case reports in dentistry · 2025Article
- Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA.Biomimetics (Basel, Switzerland) · 2024Article
- Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs.Clinical oral investigations · 2023Article
- Biocompatibility and mineralization potential of new calcium silicate cements.Journal of dental sciences · 2023Article
- Biocompatibility, bioactive potential, porosity, and interface analysis calcium silicate repair cements in a dentin tube model.Clinical oral investigations · 2023Article
- Ex Vivo Osteogenesis Induced by Calcium Silicate-Based Cement Extracts.Journal of functional biomaterials · 2023Article
- Effects of different calcium-silicate based materials on fracture resistance of immature permanent teeth with replacement root resorption and osteoclastogenesis.Restorative dentistry & endodontics · 2023Article
- Novel Osteogenic and Easily Handled Endodontic Calcium Silicate Cement Using Pluronic F127 Hydrogel.Materials (Basel, Switzerland) · 2022Article
- Biocompatibility and Osteogenic Potential of Calcium Silicate-Based Cement Combined with Enamel Matrix Derivative: Effects on Human Bone Marrow-Derived Stem Cells.Materials (Basel, Switzerland) · 2021Article
- Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers.Journal of oral biology and craniofacial researchArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium silicate-based cements differ markedly in their radiopacifiers and the presence of calcium sulfate, aluminates, carbonates and other components that can affect their biological properties. This study aimed to compare the biological properties of six calcium silicate cements in human osteoblastic cell culture (Saos-2 cells): Bio-C Repair (Bio-C), PBS HP (PBS-HP), Biodentine (Biodentine), MTA Repair HP (MTA-HP), NeoMTA Plus (NeoMTA-P), and ProRoot MTA (ProRoot). After exposure to these materials, the cells were analyzed by MTT, wound healing, cell migration, and alkaline phosphatase activity (ALP) assays, real-time PCR (qPCR) analysis of the osteogenesis markers (osteocalcin or bone gamma-carboxyglutamate protein, BGLAP; alkaline phosphatase, ALPL; bone sialoprotein or secreted phosphoprotein 1, BNSP), and alizarin red staining (ARS). Curiously, the migration rates were low 24-48 h after exposure to the materials, despite the cells showing ideal rates of viability. The advanced and intermediate cell differentiation markers BGLAP and BNSP were overexpressed in the Bio-C, MTA-HP, and ProRoot groups. Only the Biodentine group showed ALPL overexpression, a marker of initial differentiation. However, the enzymatic activity was high in all groups except Biodentine. The mineralization area was significantly large in the NeoMTA-P, ProRoot, PBS-HP, MTA-HP, and Bio-C groups. The results showed that cellular environmental stiffness, which impairs cell mobility and diverse patterns of osteogenesis marker expression, is a consequence of cement exposure. Environmental stiffness indicates chemical and physical stimuli in the microenvironment; for instance, the release of cement compounds contributes to calcium phosphate matrix formation with diverse stiffnesses, which could be essential or detrimental for the migration and differentiation of osteoblastic cells. Cells exposed to Bio-C, PBS-HP, ProRoot, NeoMTA-P, and MTA-HP seemed to enter the advanced or intermediate differentiation phases early, which is indicative of the diverse potential of cements to induce osteogenesis. Cements that quickly stimulate osteoblast differentiation may be ideal for reparative and regenerative purposes since they promptly lead to dentin or bone deposition.
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