Evidence map›Paper›PMID 34408182›Full record

ArticleScientific reports2021

DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death.

Stefano Bonassi, Marcello Ceppi, Peter Møller, Amaya Azqueta, Mirta Milić, Monica Neri, Gunnar Brunborg, Roger Godschalk, Gudrun Koppen, Sabine A S Langie and 37 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 3 pooled it
5.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 3 syntheses or guidelines pooled it, 87 citations in OpenAlex.

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  12. Application of the Comet Assay in Advanced In Vitro Models.Methods in molecular biology (Clifton, N.J.) · 2026
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

47 authors at 20 institutions in 24 countries.

Stefano BonassiUnit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma, Rome, Italy. stefano.bonassi@sanraffaele.it.
Marcello CeppiClinical Epidemiology Unit, San Martino Policlinic Hospital, Genoa, Italy.
Peter MøllerDepartment of Public Health, Section of Environmental Health, University of Copenhagen, Oster Farimagsgade 5A, 1014, Copenhagen, Denmark.
Amaya AzquetaDepartment of Pharmacology and Toxicology, University of Navarra, C/Irunlarrea 1, 31008, Pamplona, Spain.
Mirta MilićMutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia.
Monica NeriUnit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma, Rome, Italy.
Gunnar BrunborgDepartment of Environmental Health, Section of Molecular Toxicology, Norwegian Institute of Public Health (NIPH), Lovisenberggt 6, 0456, Oslo, Norway.
Roger GodschalkDepartment of Pharmacology and Toxicology, School of Nutrition and Translational Research in Metabolism, University of Maastricht, Universiteitssingel 50, 6200 MD, Maastricht, The Netherlands.
Gudrun KoppenFlemish Institute of Technological Research, Environmental Risk and Health Unit VITO - BIOMo, Mol, Belgium.
Sabine A S LangieDepartment of Pharmacology and Toxicology, School of Nutrition and Translational Research in Metabolism, University of Maastricht, Universiteitssingel 50, 6200 MD, Maastricht, The Netherlands.
João Paulo TeixeiraEnvironmental Health Department, National Institute of Health, Rua Alexandre Herculano, 321, 4000-055, Porto, Portugal.
Marco BruzzoneClinical Epidemiology Unit, San Martino Policlinic Hospital, Genoa, Italy.
Juliana Da SilvaLaboratory of Genetic Toxicology, Lutheran University of Brazil (ULBRA), and La Salle University (UNILASALLE), Canoas, RS, Brazil.
Danieli BenedettiLaboratory of Genetic Toxicology, Lutheran University of Brazil (ULBRA), and La Salle University (UNILASALLE), Canoas, RS, Brazil.
Delia CavalloDepartment of Occupational and Environmental Medicine, Epidemiology and Hygiene (DiMEILA), Italian Workers' Compensation Authority (INAIL), Via Fontana Candida 1, 00078, Monte Porzio Catone (Rome), Italy.
Cinzia Lucia UrsiniDepartment of Occupational and Environmental Medicine, Epidemiology and Hygiene (DiMEILA), Italian Workers' Compensation Authority (INAIL), Via Fontana Candida 1, 00078, Monte Porzio Catone (Rome), Italy.
Lisa GiovannelliDepartment NEUROFARBA, University of Florence, Viale G. Pieraccini 6, 50139, Florence, Italy.
Silvia MorettiDepartment of Health Sciences, Division of Dermatology, University of Florence, Palagi Hospital, Viale Michelangelo 41, Florence, Italy.
Patrizia RisoDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan, Italy.
Cristian Del Bo'Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133, Milan, Italy.
Patrizia RussoUnit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma, Rome, Italy.
Malgorzata DobrzyńskaDepartment of Radiation Hygiene and Radiobiology, National Institute of Public Health NIH - National Research Institute, 24 Chocimska Street, 00-791, Warsaw, Poland.
Irina A GoroshinskayaLaboratory for the Study of the Pathogenesis of Malignant Tumors, National Medical Research Center for Oncology, 14 line 63, 344037, Rostov-on-Don, Russia.
Ekaterina I SurikovaLaboratory for the Study of the Pathogenesis of Malignant Tumors, National Medical Research Center for Oncology, 14 line 63, 344037, Rostov-on-Don, Russia.
Marta StaruchovaInstitute of Biology, Medical Faculty, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
Magdalena BarančokovaInstitute of Biology, Medical Faculty, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
Katarina VolkovovaInstitute of Biology, Medical Faculty, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
Alena KažimirovaInstitute of Biology, Medical Faculty, Slovak Medical University, Limbova 12, 83303, Bratislava, Slovakia.
Bozena SmolkovaCancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska cesta 9, Bratislava, Slovakia.
Blanca LaffonGrupo DICOMOSA, Centro de Investigaciones Científicas Avanzadas (CICA), Departamento de Psicología, Facultad de Ciencias de La Educación, Universidade da Coruña, Campus Elviña s/n, 15071, A Coruña, Spain.
Vanessa ValdiglesiasInstituto de Investigación Biomédica de A Coruña (INIBIC), AE CICA-INIBIC, Oza, 15071, A Coruña, Spain.
Susana PastorDepartment of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès (Barcelona), Spain.
Ricard MarcosDepartment of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès (Barcelona), Spain.
Alba HernándezDepartment of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193, Cerdanyola del Vallès (Barcelona), Spain.
Goran GajskiMutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, 10000, Zagreb, Croatia.
Biljana Spremo-PotparevićCenter of Biological Research, Faculty of Pharmacy, University of Belgrade, VojvodeStepe 450, Belgrade, Serbia.
Lada ŽivkovićCenter of Biological Research, Faculty of Pharmacy, University of Belgrade, VojvodeStepe 450, Belgrade, Serbia.
Elisa Boutet-RobinetToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRAE, ENVT, INP-Purpan, UPS, Toulouse, France.
Hervé PerdryUniv Paris-Saclay, CESP, Villejuif, France.
Pierre LebaillyANTICIPE Unit, INSERM & University of Caen-Normandie Centre François Baclesse, Avenue du Général Harris, 14076, Caen Cedex 05, France.
Carlos L PerezDepartment of Biochemistry, Instituto de Ciencias Básicas Y Preclínicas "Victoria de Giron", Universidad de Ciencias Médicas de La Habana, 146 St. and 31 Ave, No, 3102, Playa, Habana, Cuba.
Nursen BasaranDepartment of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey.
Zsuzsanna NemethDepartment of Non-Ionizing Radiation, National Public Health Center, Anna Street 5, 1221, Budapest, Hungary.
Anna SafarDepartment of Non-Ionizing Radiation, National Public Health Center, Anna Street 5, 1221, Budapest, Hungary.
Maria DusinskaNILU, Health Effects Laboratory, Kjeller, Norway.
Andrew CollinsDepartment of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Sognsvannsveien 9, 0372, Oslo, Norway.
hCOMET project
Slovak Medical University · SKNorwegian Institute of Public Health · NOUniversitat Autònoma de Barcelona · ESUniversity of Oslo · NOInstitute for Medical Research and Occupational Health · HRIstituti di Ricovero e Cura a Carattere Scientifico · ITIstituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro · ITMaastricht University · NLMemorial Sloan Kettering Cancer Center · USNational Public Health and Medical Officer Service · HUOspedale Policlinico San Martino · ITRostov Research Institute of Oncology · RUUniversidade da Coruña · ESUniversidade Luterana do Brasil · BRUniversité Fédérale de Toulouse Midi-Pyrénées · FRUniversity of Belgrade · RSUniversity of Bradford · GBUniversity of Florence · ITUniversity of Milan · ITUniversity of Würzburg · DE

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
NCI NIH HHS P30 CA008748
6 · The paper itself

Abstract

The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

Indexed as

Cell-Free Nucleic AcidsComet AssayDNA DamageHumansKaplan-Meier EstimateLeukocytesNeoplasmsProportional Hazards ModelsCell-Free Nucleic Acids

Identifiers

PMID34408182
PMCPMC8373872
OpenAlexW3194225423

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.