ArticleProceedings of the National Academy of Sciences of the United States of America2021
Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification in
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 51 citations in OpenAlex.
- The RNAi machinery regulates lovastatin biosynthesis via microRNA-like RNAs in industrialSynthetic and systems biotechnology · 2026Article
- The dehydrin protein COR15 enhances antiviral RNA silencing by preventing viral protein-mediated SGS3 degradation and promoting SGS3 condensate formation.Plant communications · 2026Article
- Antiviral RNAi defective 4 (AVI4) encodes a lysophospholipid acyltransferase coopting with AVI2 and ATG8a to promote antiviral immunity in Arabidopsis.Plant communications · 2026Article
- Functional allelic diversity of PTGS components revealed by a high-throughput RUBY-based screening.The Plant journal : for cell and molecular biology · 2026Article
- Silencing Suppressor Protein p26 of Areca Palm Velarivirus 1 (APV1) Interacts With SGS3 and Promotes Its Degradation Via the Ubiquitination Pathway.Molecular plant pathology · 2026Article
- The Rice Cis-Natural Antisense Transcript NAT1850 of Pri-miR1850 Negatively Regulates Cold Tolerance by Repressing NPR3.Plant biotechnology journal · 2026Article
- Molecular basis of plant DCL4 action that outcompetes DCL2.Nature plants · 2026Article
- Advances in Plant Antiviral RNAi: From Host DCLs/RDRs to Diversified Viral Counteracting Strategies.Viruses · 2026Review
- Control of tomato brown rugose fruit virus (ToBRFV) in tomato plants using in vivo synthesized dsRNA.Journal of experimental botany · 2026Article
- RNA-binding proteins orchestrating immunity in plants.The Plant journal : for cell and molecular biology · 2025Review
- Response to "The action of Arabidopsis DICER-LIKE2 in plant growth inhibition".The Plant cell · 2025Article
- Ribosome stalling-induced NIP5;1 mRNA decay triggers ARGONAUTE1-dependent transcription downregulation.Nucleic acids research · 2025Article
- A new level of RNA-based plant protection: dsRNAs designed from functionally characterized siRNAs highly effective against Cucumber mosaic virus.Nucleic acids research · 2025Article
- Small RNA, big defence: Early epigenetic responses to genetic invasion.Quantitative plant biology · 2025Review
- RNA Metabolism and the Role of Small RNAs in Regulating Multiple Aspects of RNA Metabolism.Non-coding RNA · 2024Review
- DEAD-box RNA helicase RH20 positively regulates RNAi-based antiviral immunity in plants by associating with SGS3/RDR6 bodies.Plant biotechnology journal · 2024Article
- Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family.Biotechnology journal · 2024Article
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- The Biosynthesis Process of Small RNA and Its Pivotal Roles in Plant Development.International journal of molecular sciences · 2024Review
- Evidence for an RNAi-independent role of Arabidopsis DICER-LIKE2 in growth inhibition and basal antiviral resistance.The Plant cell · 2024Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small interfering RNAs (siRNAs) are often amplified from transcripts cleaved by RNA-induced silencing complexes (RISCs) containing a small RNA (sRNA) and an Argonaute protein. Amplified siRNAs, termed secondary siRNAs, are important for reinforcement of target repression. In plants, target cleavage by RISCs containing 22-nucleotide (nt) sRNA and Argonaute 1 (AGO1) triggers siRNA amplification. In this pathway, the cleavage fragment is converted into double-stranded RNA (dsRNA) by RNA-dependent RNA polymerase 6 (RDR6), and the dsRNA is processed into siRNAs by Dicer-like proteins. Because nonspecific RDR6 recruitment causes nontarget siRNA production, it is critical that RDR6 is specifically recruited to the target RNA that serves as a template for dsRNA formation. Previous studies showed that Suppressor of Gene Silencing 3 (SGS3) binds and stabilizes 22-nt sRNA-containing AGO1 RISCs associated with cleaved target, but how RDR6 is recruited to targets cleaved by 22-nt sRNA-containing AGO1 RISCs remains unknown. Here, using cell-free extracts prepared from suspension-cultured
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.