ArticleParasites & vectors2021
Molecular identification of Sarcocystis halieti in the muscles of two species of birds of prey from Spain.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Microscopic and Molecular Identification ofAnimals : an open access journal from MDPI · 2026Article
- Developmental stages ofBrazilian journal of veterinary medicine · 2026Article
- First report of Sarcocystis halieti in Asia: the genetic confirmation in muscles of the Eurasian sparrowhawk (Accipiter nisus) from Iran.Scientific reports · 2025Article
- Molecular Confirmation of Raptors from Spain as Definitive Hosts of NumerousAnimals : an open access journal from MDPI · 2025Article
- Morphological and Molecular Identification ofAnimals : an open access journal from MDPI · 2025Article
- Detection ofFrontiers in veterinary science · 2025Article
- The Genetic Identification of Numerous ApicomplexanAnimals : an open access journal from MDPI · 2024Article
- First report of Sarcocystis halieti (Apicomplexa) in bearded vulture (Gypaetus barbatus).Veterinary research communications · 2024Article
- Molecular identification and phylogenetic confirmation ofOpen veterinary journal · 2023Article
- Protozoan Parasites ofAnimals : an open access journal from MDPI · 2023Article
- Molecular Confirmation ofPathogens (Basel, Switzerland) · 2023Article
- A novel RFLP method for identification of morphologically similar avian Sarcocystis species.Parasitology research · 2022Article
- Article
- Role of three bird species in the life cycle of twoInternational journal for parasitology. Parasites and wildlife · 2022Article
- Investigations on Sarcocystis species in the leg muscles of the bird family Corvidae in Lithuania.Parasitology research · 2022Article
- White-tailed eagle (Frontiers in veterinary science · 2022Article
- The Role of Birds of the Family Corvidae in TransmittingAnimals : an open access journal from MDPI · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMembers of the genus Sarcocystis are protozoan parasites characterized by a prey-predator two-host life-cycle. Sarcocysts are formed in the muscles or central nervous system of the intermediate host (IH), while sporocysts develop in the small intestine of the definitive host (DH). Various birds of prey have been confirmed to be DH for Sarcocystis spp. Three Sarcocystis species, S. wobeseri, S. halieti and S. falcatula, have been identified in the muscles of birds of prey, of which the latter are known to be pathogenic and can cause encephalitis in various birds. The aim of this study was to identify Sarcocystis spp. in the muscles of birds of prey from Spain.
methodsBetween 2019 and 2020, muscle tissue samples taken from 59 birds of prey admitted to the Wildlife Recovery Centre in Ilundain (Navarra, Spain) were examined for the presence of Sarcocystis spp. Sarcocysts in fresh squashed samples were morphologically characterized under the light microscope (LM). Sarcocystis spp. were identified by means of 28S ribosomal RNA and internal transcribed spacer 1 sequence analysis.
resultsMicroscopic examination of squashed tissue samples stained with methylene blue revealed the presence of sarcocysts in three of the 59 (5.1%) birds examined. Only one sarcocyst type was observed under the LM. Sarcocysts were thread-like (1050-2160 × 130-158 μm) and had a thin (0.7-1.4 μm) and smooth cyst wall. Septa divided the cysts into compartments filled with banana-shaped (5.9 × 1.7 μm) bradyzoites. On the basis of DNA sequence results, S. halieti was identified in the western marsh harrier (Circus aeruginosus) and the black kite (Milvus migrans) for the first time. Sarcocysts of S. halieti were shorter and wider compared to those observed in the great cormorant (Phalacrocorax carbo) and the herring gull (Larus argentatus). According to current knowledge, S. halieti may infect birds belonging to four different orders: Suliformes, Charadriiformes, Strigiformes and Accipitriformes.
conclusionsThis is the first report of S. halieti in the western marsh harrier and the black kite as IH. So far, little research has been conducted on birds of prey as IH for Sarcocystis spp. These results indicate that further studies combining morphological, histopathological, and molecular methods are required.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.