ArticleClinical infectious diseases : an official publication of the Infectious Diseases Society of America2021
Diagnostic Value of 16S Ribosomal RNA Gene Polymerase Chain Reaction/Sanger Sequencing in Clinical Practice.
Article in Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- What Is the Accuracy of 16S PCR Followed by Sanger Sequencing or Next-generation Sequencing in Native Vertebral Osteomyelitis? A Systematic Review and Meta-analysis.Clinical orthopaedics and related research · 2025Pooled it
- Draft genomic sequence ofMicrobiology resource announcements · 2026Article
- Performance and Cost-Efficiency of an MPT64 Assay for DetectingJournal of Korean medical science · 2026Article
- Rapid Microbiological Diagnostics for Sepsis: Narrative Review of Current and Prospective Approaches.Critical care explorations · 2026Review
- Draft genome sequence ofMicrobiology resource announcements · 2026Article
- Etiological and Pathological Investigation of Bacterial Pneumonia in Forest Musk Deer (Veterinary medicine international · 2026Article
- Diagnostic challenges in postoperative pelvic infections associated withFrontiers in cellular and infection microbiology · 2026Article
- Wounds and the Microbiota: The Healing Interplay Between Host and Microbial Communities.International journal of molecular sciences · 2025Review
- Short Communication: Evaluation of MALDI-TOF and Sequencing Technique as Typing Tools forMicroorganisms · 2025Article
- Next Generation Sequencing Improves Diagnostic 16S rRNA Amplicon-Based Microbiota Analyses of Clinical Samples Compared to Sanger Sequencing.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2025Article
- Performance of Molecular Culture ID in diagnosis of bacterial pericarditis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- An Advanced Molecular Detection Roadmap for Nonlaboratorians.Emerging infectious diseases · 2025Article
- 16S Ribosomal RNA Gene PCR and Sequencing for Pediatric Infection Diagnosis, United States, 2020-2023.Emerging infectious diseases · 2025Article
- Actinomycosis: Mimicking Malignancies in Multiple Anatomical Sites-A Three-Patient Case Series.Medicina (Kaunas, Lithuania) · 2025Article
- The clinical impact of 16S ribosomal RNA PCR and sequencing in the identification of bacterial infections: a 7-year report from a Lebanese tertiary care center.Frontiers in cellular and infection microbiology · 2025Article
- Molecular diagnosis of campylobacter splenic abscess in an immunocompetent adolescent: A case report and literature review.IDCases · 2025Article
- Clinical Utility of Broad-Range PCR Testing and Impact on Outcomes in Adults with Suspected Infection.Antibiotics (Basel, Switzerland) · 2024Article
- Diagnostic Value of Targeted Next-generation Sequencing in Pulmonary Mycobacterial Infections.Current medical science · 2024Article
- A detailed analysis of 16S rRNA gene sequencing and conventional PCR-based testing for the diagnosis of bacterial pathogens and discovery of novel bacteria.Antonie van Leeuwenhoek · 2024Article
- Microbiological diagnosis of pleural infections: a comparative evaluation of a novel syndromic real-time PCR panel.Microbiology spectrum · 2024Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundAccurate microbiologic diagnosis is important for appropriate management of infectious diseases. Sequencing-based molecular diagnostics are increasingly used for precision diagnosis of infections. However, their clinical utility is unclear.
methodsWe conducted a retrospective analysis of specimens that underwent 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) followed by Sanger sequencing at our institution from April 2017 through March 2019.
resultsA total of 566 specimens obtained from 460 patients were studied. Patients were considered clinically infected or noninfected based on final diagnosis and management. In 17% of patients, 16S rRNA PCR/sequencing was positive and in 5% of patients, this test led to an impact on clinical care. In comparison, bacterial cultures were positive in 21% of patients. Specimens with a positive Gram stain had 12 times greater odds of having a positive molecular result than those with a negative Gram stain (95% confidence interval for odds ratio, 5.2-31.4). Overall, PCR positivity was higher in cardiovascular specimens (37%) obtained from clinically infected patients, with bacterial cultures being more likely to be positive for musculoskeletal specimens (P < .001). 16S rRNA PCR/sequencing identified a probable pathogen in 10% culture-negative specimens.
conclusion16S rRNA PCR/sequencing can play a role in the diagnostic evaluation of patients with culture-negative infections, especially those with cardiovascular infections.
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