ArticlePloS one2021
Efficacy of green synthesized silver nanoparticles via ginger rhizome extract against Leishmania major in vitro.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 37 citations in OpenAlex.
- The Effect of Elaeagnus angustifolia L. Leaf Extract on Encephalitozoon intestinalis, Acanthamoeba castellanii, and Leishmania major.Acta parasitologica · 2026Article
- Zinc nanoparticles coated with peppermint (Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2025Article
- Biogenically Synthesized Nanoparticles as Emerging Therapeutics for Leishmaniasis: A Review of Recent Advances and Potential Promises.International journal of nanomedicine · 2025Review
- Preparation, Characterization, and Leishmanicidal Assessment of Silver- and Dapsone-Loaded Niosomes Co-administration:Advanced pharmaceutical bulletin · 2024Article
- Innovating Leishmaniasis Treatment: A Critical Chemist's Review of Inorganic Nanomaterials.ACS infectious diseases · 2024Review
- Effects of silver nanoparticle based on ginger extract onArchives of Razi Institute · 2024Article
- Antileishmanial effect of podophyllotoxin and podophyllin onJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Influence of Diet on the Bioavailability of Active Components fromFoods (Basel, Switzerland) · 2023Article
- Application of Silver Nanoparticles in Parasite Treatment.Pharmaceutics · 2023Review
- Impact of Thermal Processing on the Selected Biological Activities of Ginger Rhizome-A Review.Molecules (Basel, Switzerland) · 2023Review
- The Anti-Pathogens (Basel, Switzerland) · 2023Article
- Impact of Thermal Processing on the Composition of Secondary Metabolites of Ginger Rhizome-A Review.Foods (Basel, Switzerland) · 2022Review
- Current Applications of Plant-Based Drug Delivery Nano Systems for Leishmaniasis Treatment.Pharmaceutics · 2022Review
- Article
- In Vitro Activity of Green Synthesized Silver Nanoparticles via Thymus Vulgaris Extract againstIranian journal of parasitologyArticle
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionLeishmaniasis is a major public health problem that causes by parasite of the genus Leishmania. The pentavalent antimonial compounds that used for treatment are not safe or effective enough. The aim of the present study was preparation and evaluation of the efficacy of green synthesized silver nanoparticles against Leishmania major (L. major) in vitro.
methodsTo synthesis silver (Ag) nanoparticles (NPs), ginger extract was added to the 0.2mM AgNO3 aqueous solution (1:20). Effects of different concentrations of Ag-NPs on the number of L. major promastigotes were investigated using counting assay. The MTT test was applied to determine the toxicity of Ag-NPs on promastigotes of L. major, as well as, macrophage cells. Then, to evaluate the anti-amastigotes effects of Ag-NPs, parasites within the macrophages were counted by light microscope. Furthermore, to determine the induced apoptosis and necrotic effects of Ag-NPs on promastigotes, flow cytometry method was employed using annexin staining.
resultsThe effect of Ag-NPs on promastigotes and amastigotes of L. major was effective and has a reverse relationship with its concentration. According to the results of anti-amastigote assay, the IC50 value of this nanoparticle was estimated 2.35 ppm after 72h. Also, Ag-NPs caused Programmed Cell Death (PCD) in promastigotes of L. major and showed 60.18% of apoptosis. DISCUSSION: Based on the mentioned results, it can be concluded that Ag NPs has a beneficial effect on promastigote and amastigote forms of L. major in vitro. Hence, these nanoparticles could be applied as promising antileishmanial agents for treatment of Leishmania infections.
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