Evidence map›Paper›PMID 34406853›Full record

ArticlemSphere2021

RNase III and RNase E Influence Posttranscriptional Regulatory Networks Involved in Virulence Factor Production, Metabolism, and Regulatory RNA Processing in Bordetella pertussis.

Gyles Ifill, Travis Blimkie, Amy Huei-Yi Lee, George A Mackie, Qing Chen, Scott Stibitz, Robert E W Hancock, Rachel C Fernandez

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSphere, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Determining theFrontiers in microbiology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Gyles IfillDepartment of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
Travis BlimkieDepartment of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
Amy Huei-Yi LeeDepartment of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
George A MackieDepartment of Biochemistry and Molecular Biology, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
Qing ChenDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Scott StibitzDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.
Robert E W HancockDepartment of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
Rachel C FernandezDepartment of Microbiology and Immunology, University of British Columbiagrid.17091.3e, Vancouver, British Columbia, Canada.
University of British Columbia · CACenter for Biologics Evaluation and Research · US

Funding

CIHR FDN-154287CIHR PG-53242
6 · The paper itself

Abstract

Bordetella pertussis has been shown to encode regulatory RNAs, yet the posttranscriptional regulatory circuits on which they act remain to be fully elucidated. We generated mutants lacking the endonucleases RNase III and RNase E and assessed their individual impact on the B. pertussis transcriptome. Transcriptome sequencing (RNA-Seq) analysis showed differential expression of ∼25% of the B. pertussis transcriptome in each mutant, with only 28% overlap between data sets. Both endonucleases exhibited substantial impact on genes involved in amino acid uptake (e.g., ABC transporters) and in virulence (e.g., the type III secretion system and the autotransporters

Indexed as

Bacterial ProteinsBordetella pertussisEndoribonucleasesGene Expression ProfilingGene Expression Regulation, BacterialMutationRegulonRibonuclease IIIRNA Processing, Post-TranscriptionalTranscriptomeVirulenceVirulence FactorsBacterial ProteinsEndoribonucleasesribonuclease ERibonuclease IIIVirulence FactorsBordetella pertussisposttranscriptional regulationregulatory networksRNA processingRNase ERNase IIIvirulence regulation

Identifiers

PMID34406853
PMCPMC8386462
OpenAlexW3194990160

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.