ArticleJournal of clinical microbiology2021
High Clinical Impact of Broad-Range Fungal PCR in Suspected Fungal Sinusitis.
Article in Journal of clinical microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Identification of Mycobacterium leprae and Mycobacterium lepromatosis Cases with Broad-Range Molecular Assays at a Single Large Reference Laboratory.The American journal of tropical medicine and hygiene · 2026Article
- Fungal Meningitis in US Patients Who Received Epidural Anesthesia in Matamoros, Mexico.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- Acute Invasive Fungal Rhino-Orbital Sinusitis: Current Concepts and Controversies.Clinical ophthalmology (Auckland, N.Z.) · 2026Review
- Diagnostic stewardship applied to targeted fungal sequencing.Journal of clinical microbiology · 2025Article
- Non-fungal pathogens detected by broad-range fungal polymerase chain reaction.Journal of clinical microbiology · 2025Article
- Molecular Diagnostics for Invasive Molds: From Lab to Bedside.Clinics in laboratory medicine · 2025Review
- Evaluation of metagenomic next-generation sequencing (mNGS) combined with quantitative PCR: cutting-edge methods for rapid diagnosis of non-invasive fungal rhinosinusitis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- Descriptive and molecular epidemiology of leishmaniasis diagnosed from clinical samples in the United States, 2021-2022.Microbiology spectrum · 2024Article
- Outcomes in solid organ transplant recipients receiving organs from a donor with Fusarium solani species complex meningitis.Transplant infectious disease : an official journal of the Transplantation Society · 2024Article
- The Brief Case: A case of tinea corporis caused by drug-resistantJournal of clinical microbiology · 2024Review
- Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.Journal of fungi (Basel, Switzerland) · 2024Review
- Universal PCR for bacteria, mycobacteria, and fungi: a 10-year retrospective review of clinical indications and patient outcomes.Journal of clinical microbiology · 2023Article
- Pulmonary Co-Infections Detected Premortem Underestimate Postmortem Findings in a COVID-19 Autopsy Case Series.Pathogens (Basel, Switzerland) · 2023Article
- Bartonella spp. Infections Identified by Molecular Methods, United States.Emerging infectious diseases · 2023Review
- An Unnamed Human OralCase reports in ophthalmological medicine · 2023Article
- Cokeromyces recurvatus Incidentally Found in a Patient with Gastric Outlet Obstruction.Mycopathologia · 2022Article
- Photo Quiz Answer: Orbital Apex Mass: First, Do No Harm.Journal of clinical microbiology · 2022Article
- Clinical Microbiology in 2021: My Favorite Studies about Everything Except My Least Favorite Virus.Clinical microbiology newsletter · 2022Article
- Fusariosis in Patients With Hematologic Malignancies in the Era of Antifungal Prophylaxis.Transplant infectious disease : an official journal of the Transplantation SocietyArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Broad-range fungal PCR is a powerful tool for identifying pathogens directly from patient specimens; however, reported estimates of clinical utility vary and costs discourage universal testing. We investigated the diagnostic and clinical utility of broad-range fungal PCR by examining 9 years of results from sinonasal specimens, hypothesizing that this anatomic location would identify immunocompromised patients at high risk for invasive fungal disease. We retrospectively identified 644 PCRs and 1,446 fungal cultures from sinus sites. To determine the relative performance of each testing modality, we performed chart review on 52 patients having specimens submitted for culture and PCR on the same day. Positivity rates were significantly higher for PCR (37.1%) than culture (13.7%) but similar for formalin-fixed and fresh tissues (42.3% versus 34.6%). Relative to culture, PCR had significantly faster turnaround time to both preliminary (94.5 versus 108.8 h) and final positive (137.9 versus 278.5 h) results. Among chart-reviewed patients, 88% were immunocompromised, 65% had proven or probable fungal disease, and testing sensitivities for culture and PCR (67.5% and 85.0%) were not statistically different. Nevertheless, PCR identified pathogens not recovered by culture in 14.9% of cases and informed clinical decision-making in 16.7% of all reviewed cases, and sensitivity of PCR combined with culture (90.0%) was higher than that of culture alone. We conclude that broad-range fungal PCR is frequently informative for patients at risk of serious fungal disease and is complementary to and has faster turnaround time than culture. Formalin-fixed tissue does not adversely affect diagnostic yield, but anatomic site may impact assay positivity rates.
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Registered trials
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