ReviewCytometry. Part A : the journal of the International Society for Analytical Cytology2022
Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants.
Review in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Development of a low-coverage whole genome sequencing screen for apomixis using a diverse set of Malus germplasm.PLoS genetics · 2026Article
- Distinct genomic architectures but the same gene underlie the convergent evolution of a plant supergene.Science advances · 2026Article
- Multi-dimensional identification and quality evaluation ofFrontiers in plant science · 2026Article
- Commentary: Re-calibration of flow cytometry standards for plant genome size estimation.Frontiers in plant science · 2026Article
- The winner takes it all: a single genotype of Kalanchoe × houghtonii is a global invader.Annals of botany · 2025Article
- Genome Survey Sequencing ofGenes · 2025Article
- ZmSSRP1 facilitates the progression of RNA polymerase II and is essential for kernel development in maize.The Plant cell · 2025Article
- Induction of stigma-like structures in saffron (Crocus sativus L.): Exploring factors and metabolite analysis.PloS one · 2025Article
- Re-calibration of flow cytometry standards for plant genome size estimation.Frontiers in plant science · 2025Article
- CAM evolution is associated with gene family expansion in an explosive bromeliad radiation.The Plant cell · 2024Article
- Leaf Architecture and Genome Size Variation ofTropical life sciences research · 2024Article
- Genome Size in thePlants (Basel, Switzerland) · 2024Article
- LocoGSE, a sequence-based genome size estimator for plants.Frontiers in plant science · 2024Article
- Does the evolution of micromorphology accompany chromosomal changes on dysploid and polyploid levels in the Barnardia japonica complex (Hyacinthaceae)?BMC plant biology · 2023Article
- Genome Size Variation across a Cypriot Fabeae Tribe Germplasm Collection.Plants (Basel, Switzerland) · 2023Article
- Mastering DNA Content Estimation by Flow Cytometry as an Efficient Tool for Plant Breeding and Biodiversity Research.Methods and protocols · 2023Article
- Complex patterns of ploidy in a holocentric plant clade (Schoenus, Cyperaceae) in the Cape biodiversity hotspot.Annals of botany · 2023Article
- Cryptic invasion suggested by a cytogeographic analysis of the halophyticFrontiers in plant science · 2023Article
- Chromosome size matters: genome evolution in the cyperid clade.Annals of botany · 2022Article
- Fern Spores-"Ready-to-Use" Standards for Plant Genome Size Estimation Using a Flow Cytometric Approach.Plants (Basel, Switzerland) · 2022Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The estimation of nuclear DNA content has been by far the most popular application of flow cytometry in plants. Because flow cytometry measures relative fluorescence intensities of nuclei stained by a DNA fluorochrome, ploidy determination, and estimation of the nuclear DNA content in absolute units both require comparison to a reference standard of known DNA content. This implies that the quality of the results obtained depends on the standard selection and use. Internal standardization, when the nuclei of an unknown sample and the reference standard are isolated, stained, and measured simultaneously, is mandatory for precise measurements. As DNA peaks representing G
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