Evidence map›Paper›PMID 34404904›Full record

ArticleCommunications biology2021

Integrated multi-omics analysis of RB-loss identifies widespread cellular programming and synthetic weaknesses.

Swetha Rajasekaran, Jalal Siddiqui, Jessica Rakijas, Brandon Nicolay, Chenyu Lin, Eshan Khan, Rahi Patel, Robert Morris, Emanuel Wyler, Myriam Boukhali and 10 more

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors.

Swetha RajasekaranDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.ORCID http://orcid.org/0000-0002-1271-6204
Jalal SiddiquiDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.
Jessica RakijasDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.
Brandon NicolayMassachusetts General Hospital Cancer Center, Charlestown, MA, USA.
Chenyu LinDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.
Eshan KhanDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.
Rahi PatelDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.
Robert MorrisMassachusetts General Hospital Cancer Center, Charlestown, MA, USA.
Emanuel WylerBerlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.ORCID http://orcid.org/0000-0002-9884-1806
Myriam BoukhaliMassachusetts General Hospital Cancer Center, Charlestown, MA, USA.
Jayashree BalasubramanyamDepartment of Nanobiotechnology, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India.
R Ranjith KumarDepartment of Nanobiotechnology, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India.
Capucine Van RechemDepartment of Pathology, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-5408-6124
Christine VogelCenter for Genomics and Systems Biology, Department of Biology, New York University, New York, USA.
Sailaja V ElchuriDepartment of Nanobiotechnology, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India.
Markus LandthalerBerlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.ORCID http://orcid.org/0000-0002-1075-8734
Benedikt ObermayerBerlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.
Wilhelm HaasMassachusetts General Hospital Cancer Center, Charlestown, MA, USA.
Nicholas DysonMassachusetts General Hospital Cancer Center, Charlestown, MA, USA. dyson@helix.mgh.harvard.edu.
Wayne MilesDepartment of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA. wayne.miles@osumc.edu.ORCID http://orcid.org/0000-0002-2054-5206

Funding

Translational Therapeutics Research Program (TT)P30CA016058 · NCI · OHIO STATE UNIVERSITY · PI Tasleem J Padamsee · 1985 to 2026
$132.3M
Next generation gene expression analysisR35GM127089 · NIGMS · NEW YORK UNIVERSITY · PI Christine Vogel · 2018 to 2026
$4.0M
The consequences of RB-lossR01GM117413 · NIGMS · MASSACHUSETTS GENERAL HOSPITAL · PI DYSON, NICHOLAS J · 2016 to 2019
$2.1M
The translation regulation of pro-apoptotic genesR01CA251753 · NCI · OHIO STATE UNIVERSITY · PI MILES, WAYNE · 2020 to 2024
$2.0M
Interplay between transcription and translationK22CA204352 · NCI · OHIO STATE UNIVERSITY · PI MILES, WAYNE · 2016 to 2018
$561k
NCI NIH HHS K22 CA204352NCI NIH HHS P30 CA016058NCI NIH HHS R01 CA251753NIGMS NIH HHS R01 GM117413NIGMS NIH HHS R35 GM127089
6 · The paper itself

Abstract

Inactivation of RB is one of the hallmarks of cancer, however gaps remain in our understanding of how RB-loss changes human cells. Here we show that pRB-depletion results in cellular reprogramming, we quantitatively measured how RB-depletion altered the transcriptional, proteomic and metabolic output of non-tumorigenic RPE1 human cells. These profiles identified widespread changes in metabolic and cell stress response factors previously linked to E2F function. In addition, we find a number of additional pathways that are sensitive to RB-depletion that are not E2F-regulated that may represent compensatory mechanisms to support the growth of RB-depleted cells. To determine whether these molecular changes are also present in RB1

Indexed as

AnimalsCell Line, TumorHumansMiceRetinal NeoplasmsRetinoblastomaRetinoblastoma Binding ProteinsUbiquitin-Protein LigasesRB1 protein, humanRb1 protein, mouseRetinoblastoma Binding ProteinsUbiquitin-Protein Ligases

Identifiers

PMID34404904
PMCPMC8371045

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.