ArticleNature communications2021
Genome-scale target identification in Escherichia coli for high-titer production of free fatty acids.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 94 citations in OpenAlex.
- Reprogramming energy system for growth and bioproduction from CO₂ and methanol in Escherichia coli.Nature communications · 2026Article
- Identifying metabolic bottlenecks for micafungin precursor production via untargeted regulatory perturbation.Biotechnology for biofuels and bioproducts · 2026Article
- Recent advances in microbial production of odd-chain fatty acids.World journal of microbiology & biotechnology · 2026Review
- Multilayer regulation of CRISPR systems: integrating anti-CRISPR proteins, CRISPRi/a, and quorum sensing networks.Frontiers in cellular and infection microbiology · 2026Review
- Engineering controllable alteration of malonyl-CoA levels to enhance polyketide production.Nature chemical biology · 2025Article
- Establishment of CRISPR-STAR System to Realise Simultaneous Transcriptional Activation and Repression in Yarrowia lipolytica.Microbial biotechnology · 2025Article
- Genome-scale CRISPRi screen identifies pcnB repression conferring improved physiology for overproduction of free fatty acids in Escherichia coli.Nature communications · 2025Article
- Comprehensive evaluation of the capacities of microbial cell factories.Nature communications · 2025Article
- A rational multi-target combination strategy for synergistic improvement of non-ribosomal peptide production.Nature communications · 2025Article
- De novo biosynthesis of nylon 12 monomer ω-aminododecanoic acid.Nature communications · 2025Article
- CRISPRi-mediated metabolic switch enables concurrent aerobic and synthetic anaerobic fermentations in engineered consortium.Nature communications · 2024Article
- The rise and future of CRISPR-based approaches for high-throughput genomics.FEMS microbiology reviews · 2024Review
- Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling.Nature communications · 2024Article
- Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources.Journal of microbiology (Seoul, Korea) · 2024Article
- Recent advances in genome-scale engineering inEngineering microbiology · 2024Review
- Identification of crucial roles of transcription factor IhfA on high production of free fatty acids inSynthetic and systems biotechnology · 2024Article
- The expanded CRISPR toolbox for constructing microbial cell factories.Trends in biotechnology · 2024Review
- Budding and explosive membrane vesicle production by hypervesiculatingFrontiers in microbiology · 2024Article
- Genetic Engineering of Microorganisms with Electroactive Genes for the Fabrication of Electrochemical Microbial Biosensors.Methods in molecular biology (Clifton, N.J.) · 2024Article
- An improved CRISPRi system inSynthetic and systems biotechnology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To construct a superior microbial cell factory for chemical synthesis, a major challenge is to fully exploit cellular potential by identifying and engineering beneficial gene targets in sophisticated metabolic networks. Here, we take advantage of CRISPR interference (CRISPRi) and omics analyses to systematically identify beneficial genes that can be engineered to promote free fatty acids (FFAs) production in Escherichia coli. CRISPRi-mediated genetic perturbation enables the identification of 30 beneficial genes from 108 targets related to FFA metabolism. Then, omics analyses of the FFAs-overproducing strains and a control strain enable the identification of another 26 beneficial genes that are seemingly irrelevant to FFA metabolism. Combinatorial perturbation of four beneficial genes involving cellular stress responses results in a recombinant strain ihfA
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