ArticlePLoS genetics2021
Single-cell RNA-sequencing reveals pre-meiotic X-chromosome dosage compensation in Drosophila testis.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 50 citations in OpenAlex.
- Single-cell consequences of X-linked meiotic drive in stalk-eyed flies.PLoS genetics · 2025Article
- Single cell RNA-sequencing reveals no evidence for meiotic sex chromosome inactivation in the threespine stickleback fish.PLoS genetics · 2025Article
- Paralog-Dependent Specialization of Paf1C Subunit, Ctr9, for Sex Chromosome Gene Regulation and Male Germline Differentiation in Drosophila.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Single cell RNA-sequencing reveals no evidence for meiotic sex chromosome inactivation in the threespine stickleback fish.bioRxiv : the preprint server for biology · 2025Article
- Comparative single-cell analysis of transcriptional bursting reveals the role of genome organization in de novo transcript origination.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quantifying Transcriptome Turnover on Phylogenies by Modeling Gene Expression as a Binary Trait.Molecular biology and evolution · 2025Article
- Comparative single cell analysis of transcriptional bursting reveals the role of genome organization onbioRxiv : the preprint server for biology · 2025Article
- Dynamics of X chromosome hyper-expression and inactivation in male tissues during stick insect development.PLoS genetics · 2025Article
- Sex Chromosome Dosage Compensation in Insects.Insects · 2025Review
- A noncanonical role of roX RNAs in autosomal epigenetic repression.Nature communications · 2025Article
- Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome.PLoS genetics · 2024Article
- Single-cell RNA-seq of Drosophila miranda testis reveals the evolution and trajectory of germline sex chromosome regulation.PLoS biology · 2024Article
- scRNA-seq Reveals Novel Genetic Pathways and Sex Chromosome Regulation in Tribolium Spermatogenesis.Genome biology and evolution · 2024Article
- Article
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- Paralog transcriptional differentiation in the D. melanogaster-specific gene family Sdic across populations and spermatogenesis stages.Communications biology · 2023Article
- Chromosome-specific maturation of the epigenome in the Drosophila male germline.bioRxiv : the preprint server for biology · 2023Article
- Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.Communications biology · 2023Article
- Article
- VCP promotes tTAF-target gene expression and spermatocyte differentiation by downregulating mono-ubiquitylated H2A.Development (Cambridge, England) · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Dosage compensation equalizes X-linked expression between XY males and XX females. In male fruit flies, expression levels of the X-chromosome are increased approximately two-fold to compensate for their single X chromosome. In testis, dosage compensation is thought to cease during meiosis; however, the timing and degree of the resulting transcriptional suppression is difficult to separate from global meiotic downregulation of each chromosome. To address this, we analyzed testis single-cell RNA-sequencing (scRNA-seq) data from two Drosophila melanogaster strains. We found evidence that the X chromosome is equally transcriptionally active as autosomes in somatic and pre-meiotic cells, and less transcriptionally active than autosomes in meiotic and post-meiotic cells. In cells experiencing dosage compensation, close proximity to MSL (male-specific lethal) chromatin entry sites (CES) correlates with increased X chromosome transcription. We found low or undetectable levels of germline expression of most msl genes, mle, roX1 and roX2 via scRNA-seq and RNA-FISH, and no evidence of germline nuclear roX1/2 localization. Our results suggest that, although dosage compensation occurs in somatic and pre-meiotic germ cells in Drosophila testis, there might be non-canonical factors involved in the dosage compensation mechanism. The single-cell expression patterns and enrichment statistics of detected genes can be explored interactively in our database: https://zhao.labapps.rockefeller.edu/gene-expr/.
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