ArticleTropical animal health and production2021
Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan.
Article in Tropical animal health and production, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 32 citations in OpenAlex.
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- Molecular Investigation of Anaplasma spp. and Genotype Profile of A. ovis in Sheep from Different Farms in Türkiye.Acta parasitologica · 2025Article
- Molecular epidemiology and genetic diversity of Anaplasma and Theileria spp. in Pakistani sheep.PloS one · 2025Article
- Pakistan sheep industry its constrains and future trends.Tropical animal health and production · 2024Review
- Leading report of molecular prevalence of tick borne Anaplasma marginale and Theileria ovis in yaks (Bos grunniens) from Pakistan.Archives of microbiology · 2024Article
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- Tick-borne haemoparasitic diseases in small ruminants in Pakistan: Current knowledge and future perspectives.Saudi journal of biological sciences · 2022Review
- Prevalence ofSaudi journal of biological sciences · 2022Article
- Prevalence, epidemiology, seasonality, and phylogeny of Anaplasma marginale in blood samples of goats collected from Punjab, Pakistan.Tropical animal health and production · 2022Article
- Molecular evidence, risk factors analysis, and hematological alterations associated withIranian journal of veterinary research · 2022Article
- Molecular Epidemiology ofAnimals : an open access journal from MDPI · 2021Article
- Molecular Characterization ofIranian journal of parasitologyArticle
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Theileria ovis and Anaplasma marginale are intracellular pathogens affecting a wide range of animals, causing huge economic losses worldwide. The present study reports the molecular evidence of Theileria ovis and Anaplasma marginale in sheep blood samples (N = 218) collected from Layyah district in Punjab (Pakistan), where economy heavily relies on livestock. A 520 base pair fragment specific for 18S ribosomal RNA gene of Theileria ovis was PCR amplified in 23/218 (10.6%) sheep blood samples, while for Anaplasma marginale, a 265 base pair fragment specific for msp1b gene was generated in 15/218 (6.9%) sheep blood samples. Two blood samples were found co-infected (0.9%) with both parasites. Amplified PCR products of both parasites were confirmed by DNA sequencing and submitted to GenBank. Prevalence of both Theileria ovis (p = 0.3) and Anaplasma marginale (p = 0.4) varied non-significantly among the investigated sheep breeds. Tick burden on dogs present with sheep herds was found associated with Theileria ovis infection in sheep (p = 0.05). It was observed that lambs (p = 0.009), sheep in small herds (p = 0.04), and tick burden on dogs present with sheep herds (p = 0.01) were associated with Anaplasma marginale infection in sheep during the present study. In conclusion, we are reporting a higher prevalence of Theileria ovis than Anaplasma marginale in blood samples of sheep collected from Layyah district. Tick-infested dogs were found to be risk factors for the transmission of both pathogens in sheep, and tick control strategies should be extended to dogs associated with sheep herds in this area.
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