Evidence map›Paper›PMID 34399682›Full record

ArticleCellular & molecular biology letters2021

Apoptosis-promoting properties of miR-3074-5p in MC3T3-E1 cells under iron overload conditions.

Yi Feng, Pei-Yan He, Wei-Dong Kong, Wan-Jing Cen, Peng-Lin Wang, Chang Liu, Wu Zhang, Shu-Shu Li, Jian-Wei Jiang

Open access · goldAbstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular & molecular biology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 38 citations in OpenAlex.

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  18. STAT3-mediated osteogenesis and osteoclastogenesis in osteoporosis.Cell communication and signaling : CCS · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Yi Feng *Department of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China.
Pei-Yan He *Department of Biochemistry, Basic Medical College, Jinan University, No.601 Huangpu Road West, Guangzhou, 510632, China.
Wei-Dong KongDepartment of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China.
Wan-Jing CenDepartment of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China.
Peng-Lin WangDepartment of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China.
Chang LiuDepartment of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China.
Wu ZhangDepartment of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China.
Shu-Shu LiDepartment of Orthodontics, The First Affiliated Hospital of Jinan University, No.613 Huangpu Road West, Guangzhou, 510630, China. lishushu1987@126.com.
Jian-Wei JiangDepartment of Biochemistry, Basic Medical College, Jinan University, No.601 Huangpu Road West, Guangzhou, 510632, China. jjw703@jnu.edu.cn.
First Affiliated Hospital of Jinan University · CNJinan University · CN

Funding

science and technology planning project of guangdong province 2015A020214015science and technology planning project of guangdong province 2016A020220015science and technology planning project of guangzhou, china 201904010145
6 · The paper itself

Abstract

backgroundIron overload can promote the development of osteoporosis by inducing apoptosis in osteoblasts. However, the mechanism by which miRNAs regulate apoptosis in osteoblasts under iron overload has not been elucidated.

methodThe miRNA expression profile in MC3T3-E1 cells under iron overload was detected by next generation sequencing. qRT-PCR was used to determine the expression of miR-3074-5p in MC3T3-E1 cells under iron overload. The proliferation of MC3T3-E1 cells was tested using CCK-8 assays, and apoptosis was measured using flow cytometry. The miRanda and TargetScan databases were used to predict the target genes of miR-3074-5p. Interaction between miR-3074-5p and the potential target gene was validated by qRT-PCR, luciferase reporter assay and western blotting.

resultsWe found that iron overload decreased the cell viability and induced apoptosis of MC3T3-E1 cells. The results of next generation sequencing analysis showed that miR-3074-5p expression was significantly increased in MC3T3-E1 cells under iron overload conditions, which was confirmed by further experiments. The inhibition of miR-3074-5p attenuated the apoptosis of iron-overloaded MC3T3-E1 cells. Furthermore, the expression of Smad4 was decreased and was inversely correlated with miR-3074-5p expression, and overexpression of Smad4 partially reversed the viability inhibition of iron-overloaded MC3T3-E1 cells by relieving the suppression of ERK, AKT, and Stat3 phosphorylation, suggesting its regulatory role in the viability inhibition of iron-overloaded MC3T3-E1 cells. The luciferase reporter assay results showed that Smad4 was the target gene of miR-3074-5p.

conclusionmiR-3074-5p functions as an apoptosis promoter in iron-overloaded MC3T3-E1 cells by directly targeting Smad4.

Indexed as

AnimalsApoptosisCell LineIron OverloadMiceMicroRNAsOsteoblastsSignal TransductionSmad4 ProteinMicroRNAsSmad4 ProteinSmad4 protein, mouseApoptosisIron overloadMC3T3-E1 cellsmiRNA-3074-5pOsteoporosis

Identifiers

PMID34399682
PMCPMC8365891
OpenAlexW3196191694

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.