Evidence map›Paper›PMID 34398899›Full record

ArticlePloS one2021

Active rheumatoid arthritis in a mouse model is not an independent risk factor for periprosthetic joint infection.

Rishi Trikha, Danielle Greig, Troy Sekimura, Nicolas Cevallos, Benjamin Kelley, Zeinab Mamouei, Christopher Hart, Micah Ralston, Amr Turkmani, Adam Sassoon and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. The 2023 Orthopaedic Research Society's International Consensus Meeting on musculoskeletal infection: Summary from the host immunity section.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Rishi TrikhaUniversity of California, Los Angeles, Los Angeles, CA, United States of America.ORCID 0000-0003-4193-5446
Danielle GreigUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Troy SekimuraUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Nicolas CevallosUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Benjamin KelleyUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Zeinab MamoueiUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Christopher HartUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Micah RalstonUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Amr TurkmaniUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Adam SassoonUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Alexandra StavrakisUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
Nicholas M BernthalUniversity of California, Los Angeles, Los Angeles, CA, United States of America.
University of California, Los Angeles · US

Funding

Regenerative Musculoskeletal Medicine Training ProgramT32AR059033 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI John S Adams, Nicholas M. Bernthal · 2011 to 2026
$4.8M
Modifying and Delivering Antimicrobials to Prevent Orthopaedic Implant InfectionsK08AR069112 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BERNTHAL, NICHOLAS M. · 2016 to 2019
$704k
NIAMS NIH HHS K08 AR069112NIAMS NIH HHS T32 AR059033
6 · The paper itself

Abstract

introductionPeriprosthetic joint infection (PJI) represents a devastating complication of total joint arthroplasty associated with significant morbidity and mortality. Literature suggests a possible higher incidence of periprosthetic joint infection (PJI) in patients with rheumatoid arthritis (RA). There is, however, no consensus on this purported risk nor a well-defined mechanism. This study investigates how collagen-induced arthritis (CIA), a validated animal model of RA, impacts infectious burden in a well-established model of PJI.

methodsControl mice were compared against CIA mice. Whole blood samples were collected to quantify systemic IgG levels via ELISA. Ex vivo respiratory burst function was measured via dihydrorhodamine assay. Ex vivo Staphylococcus aureus Xen36 burden was measured directly via colony forming unit (CFU) counts and crystal violet assay to assess biofilm formation. In vivo, surgical placement of a titanium implant through the knee joint and inoculation with S. aureus Xen36 was performed. Bacterial burden was then quantified by longitudinal bioluminescent imaging.

resultsMice with CIA demonstrated significantly higher levels of systemic IgG compared with control mice (p = 0.003). Ex vivo, there was no significant difference in respiratory burst function (p = 0.89) or S. aureus bacterial burden as measured by CFU counts (p = 0.91) and crystal violet assay (p = 0.96). In vivo, no significant difference in bacterial bioluminescence between groups was found at all postoperative time points. CFU counts of both the implant and the peri-implant tissue were not significantly different between groups (p = 0.82 and 0.80, respectively).

conclusionThis study demonstrated no significant difference in S. aureus infectious burden between mice with CIA and control mice. These results suggest that untreated, active RA may not represent a significant intrinsic risk factor for PJI, however further mechanistic translational and clinical studies are warranted.

Indexed as

Arthritis, ExperimentalArthroplasty, Replacement, KneeBone-Implant InterfaceKnee JointStaphylococcal InfectionsAnimalsBacterial LoadKnee ProsthesisMaleMiceRisk FactorsStaphylococcus aureus

Identifiers

PMID34398899
PMCPMC8366981
OpenAlexW3193770459

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.