ArticleReproduction (Cambridge, England)2021
Effect of in vitro growth on mouse oocyte competency, mitochondria and transcriptome.
Article in Reproduction (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 18 citations in OpenAlex.
- Ovarian and follicular culture enables the production of fertile oocytes in C57BL/6 mice.The Journal of reproduction and development · 2026Article
- Niacin improves developmental ability by increasing glutathione levels and promoting male pronuclear formation in porcine oocytes grown in vitro.The Journal of reproduction and development · 2026Article
- Production and Use of Customizable Agarose Molds for Scaffold-Free Mouse Ovarian Follicle Culture.Journal of visualized experiments : JoVE · 2025Article
- Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro.Reproduction (Cambridge, England) · 2025Article
- Comprehensive atlas of mitochondrial distribution and dynamics during oocyte maturation in mouse models.Biomarker research · 2024Article
- Human Umbilical Cord Mesenchymal Stem Cells Derived Exosomes Improved The Aged Mouse IVM Oocytes Quality.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Regulation of Oocyte mRNA Metabolism: A Key Determinant of Oocyte Developmental Competence.Advances in anatomy, embryology, and cell biology · 2024Review
- Pronuclear transfer rescues poor embryo development ofHuman reproduction open · 2024Article
- High-quality single-cell transcriptomics from ovarian histological sections during folliculogenesis.Life science alliance · 2023Article
- From in vivo to in vitro: exploring the key molecular and cellular aspects of human female gametogenesis.Human cell · 2023Review
- MitoQ Protects Ovarian Organoids against Oxidative Stress during Oogenesis and Folliculogenesis In Vitro.International journal of molecular sciences · 2023Article
- Prospects for fertility preservation: the ovarian organ function reconstruction techniques for oogenesis, growth and maturationFrontiers in physiology · 2023Review
- Mitochondrial DNA mutations can influence the post-implantation development of human mosaic embryos.Frontiers in cell and developmental biology · 2023Article
- Oocyte quality following in vitro follicle development†.Biology of reproduction · 2022Review
- Current Status of In Vitro Oocyte Growth and Development in Mammals.Reproductive medicine and biologyReview
- TFAM-Mediated mtDNA Replication is Essential for Developmental Competence of In Vitro Grown Oocytes.Reproductive medicine and biologyArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro generation of fertile oocytes has been reported in several mammalian species. However, oocyte integrity is compromised by in vitro culture. Here, we aimed to understand the factors affecting oocyte competency by evaluating mitochondrial function and transcriptome as well as lipid metabolism in in vivo-derived oocytes and in vitro grown and matured (IVGM) oocytes under atmospheric (20%) and physiological (7%) O2 concentration. We used single-cell RNA-sequencing as well as Gene Ontology and KEGG analyses to identify the molecular pathways affecting the developmental competence of oocytes. Oocytes grown under 20% O2 conditions showed a significant decrease in mitochondrial membrane potential, upregulation of ceramide synthesis pathway-associated genes, and high ceramide accumulation compared with oocytes grown under 7% O2 conditions and in vivo-grown oocytes. This suggests that excess ceramide level causes mitochondrial dysfunction and poor developmental ability of the oocytes. Mitochondrial DNA copy number was lower in IVGM oocytes irrespective of O2 concentration in culture, although there was no common abnormality in the expression of genes related to mitochondrial biosynthesis. In contrast, some oocytes produced under 7% O2 conditions showed gene expression profiles similar to those of in vivo-grown oocytes. In these oocytes, the expression of transcription factors, including Nobox, was restored. Nobox expression correlated with the expression of genes essential for oocyte development. Thus, Nobox may contribute to the establishment of oocyte competency before and after the growth phase. The comprehensive analysis of IVGM oocytes presented here provides a platform for elucidating the mechanism underlying functional oocyte production in vivo.
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