Evidence map›Paper›PMID 34395756›Full record

ArticleBio-protocol2018

BMV Propagation, Extraction and Purification Using Chromatographic Methods.

Aleksander Strugała, Paulina Bierwagen, Jakub Dalibor Rybka, Michał Giersig, Marek Figlerowicz, Anna Urbanowicz

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Aleksander StrugałaInstitute of Bioorganic Chemistry, Polish Academy of Science, Poznań, Poland.
Paulina BierwagenInstitute of Bioorganic Chemistry, Polish Academy of Science, Poznań, Poland.
Jakub Dalibor RybkaCenter of Advanced Technologies, Adam Mickiewicz University, Poznań, Poland.
Michał GiersigCenter of Advanced Technologies, Adam Mickiewicz University, Poznań, Poland.
Marek FiglerowiczInstitute of Bioorganic Chemistry, Polish Academy of Science, Poznań, Poland.
Anna UrbanowiczInstitute of Bioorganic Chemistry, Polish Academy of Science, Poznań, Poland.
Institute of Bioorganic Chemistry, Polish Academy of Sciences · PLAdam Mickiewicz University in Poznań · PLPoznań University of Technology · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and size-exclusion chromatography. The presented method is of similar efficiency to previously described protocols relying on differential centrifugation and can easily be scaled up. The resulting BMV capsids are stable and monodisperse and can be used for further applications.

Indexed as

BMVBrome mosaic virusCapsidPlant virus purificationVirionVirus-like particles (VLP)

Identifiers

PMID34395756
PMCPMC8328587
OpenAlexW2885014583

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.