ArticleBio-protocol2018
Mouse Mammary Gland Whole Mount Preparation and Analysis.
Article in Bio-protocol, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 28 citations in OpenAlex.
- Plant-Based α-Linolenic Acid as a Superior Nutraceutical to Modulates Apoptosis, Hypoxic Signaling, and Lipid Metabolic Pathway in Breast Cancer: A Comparative Preclinical Study.Molecular nutrition & food research · 2026Article
- Tumor sialylation regulates G-CSF stability and promotes neutrophil-mediated immunosuppression in breast cancer.Nature communications · 2026Article
- Lentinula edodes Cultured Extract Intake at Puberty Mitigates Inflammatory Signals at the Mammary Glands by the Involvement of Epigenetic Mechanisms in BALB/c Mice.The breast journal · 2026Article
- Isolation of regulatory T cells from healthy murine mammary glands.Methods in cell biology · 2026Article
- Luminal hormone-responsive cells tune the regenerative remodeling of mammary glands in large mammals.Cell discovery · 2025Article
- The class A repeats of LRP5 are required for normal development of bone, retinal vasculature and mammary gland in vivo.Disease models & mechanisms · 2025Article
- DEK promotes mammary hyperplasia and is associated with H3K27me3 epigenetic modifications.Life science alliance · 2025Article
- Adipocyte-specific Zeb1 downregulation remodels the tumor-associated adipose microenvironment to facilitate female breast cancer progression.Nature communications · 2025Article
- Methods for Imaging Intracellular Calcium Signals in the Mouse Mammary Epithelium in Two and Three Dimensions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Whole mount preparation and analysis of rabbit mammary gland.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- Article
- Cancer cell stiffening via CoQNature communications · 2024Article
- C-terminally phosphorylated p27 activates self-renewal driver genes to program cancer stem cell expansion, mammary hyperplasia and cancer.Nature communications · 2024Article
- USP22 supports the aggressive behavior of basal-like breast cancer by stimulating cellular respiration.Cell communication and signaling : CCS · 2024Article
- A long-acting prolactin to combat lactation insufficiency.bioRxiv : the preprint server for biology · 2023Article
- Characterization of Mammary Tumors Arising from MMTV-PyVT Transgenic Mice.Current issues in molecular biology · 2023Article
- Effect of Maternal Gradient Nutritional Restriction during Pregnancy on Mammary Gland Development in Offspring.Animals : an open access journal from MDPI · 2023Article
- Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing.The American journal of pathology · 2022Article
- Best practices to quantify the impact of reproductive toxicants on development, function, and diseases of the rodent mammary gland.Reproductive toxicology (Elmsford, N.Y.) · 2022Review
- Inflammation-induced PELP1 expression promotes tumorigenesis by activating GM-CSF paracrine secretion in the tumor microenvironment.The Journal of biological chemistry · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure a method is needed that provides an overview of the entire gland. Whole mounts provide this information, are inexpensive and do not require specialized equipment. This protocol describes mammary gland isolation, whole mount preparation and analysis. Mammary gland tissue, which is removed postmortem, is stained with Carmine Alum, a nuclear stain, allowing detection of epithelial structures embedded in the adipose tissue of the mammary fat pad. Stained mammary glands are imaged by light microscopy or embedded and sectioned for histological examination. Image analysis software such as Image J can be used to quantify extensity of branching complexity, epithelial structure remodeling or hyperplastic changes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.