Evidence map›Paper›PMID 34395724›Full record

ArticleBio-protocol2021

Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.

Corey R Landry, Mighten C Yip, Ilya Kolb, William A Stoy, Mercedes M Gonzalez, Craig R Forest

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Corey R LandryWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, USA.
Mighten C YipGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA.
Ilya KolbJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, USA.
William A StoyNeuroTechnology Center, Columbia University, New York, USA.
Mercedes M GonzalezGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA.
Craig R ForestGeorge W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA.

Funding

Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7M
High-throughput robotic analysis of integrated neuronal phenotypesR01EY023173 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2012 to 2016
$4.3M
Scalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2M
In-vivo circuit activity measurement at single cell, sub-threshold resolutionU01MH106027 · NIMH · GEORGIA INSTITUTE OF TECHNOLOGY · PI FOREST, CRAIG, STANLEY, GARRETT B. · 2014 to 2016
$1.5M
NEI NIH HHS R01 EY023173NIDA NIH HHS R01 DA029639NIMH NIH HHS U01 MH106027NINDS NIH HHS R01 NS102727
6 · The paper itself

Abstract

The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and automation were limited by the need to replace pipettes manually after each experiment. This article presents an optimized protocol for pipette cleaning, which enables it to be performed quickly (< 30 s), resulting in a high yield of whole-cell recording success rate (> 90%) for over 100 reuses of a single pipette. For most patch-clamp experiments (< 30 whole-cell recordings per day), this method enables a single pipette to be used for an entire day of experiments. In addition, we describe easily implementable hardware and software as well as troubleshooting tips to help other labs implement this method in their own experiments. Pipette cleaning enables patch-clamp experiments to be performed with higher throughput, whether manually or in an automated fashion, by eliminating the tedious and skillful task of replacing pipettes. From our experience with numerous electrophysiology laboratories, pipette cleaning can be integrated into existing patch-clamp setups in approximately one day using the hardware and software described in this article. Graphic abstract: Rapid enzymatic cleaning for reuse of patch-clamp pipettes.

Indexed as

AutomationElectrophysiologyEnzymatic detergentHigh-throughputPatch-clamp

Identifiers

PMID34395724
PMCPMC8329470

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.