ArticleJournal of Indian Society of Periodontology
Isolation and immunohistochemical characterization of periodontal ligament stem cells: A preliminary study.
Article in Journal of Indian Society of Periodontology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- In Vitro Evaluation of the Regenerative Potential of Autologous Platelet-Rich Fibrin (PRF) on Human Primary Periodontal Ligament Cells.International journal of molecular sciences · 2025Article
- The Combination of Biomimetic Materials Enhances the Functional Differentiation of Stem Cells Exfoliated from Human Deciduous Teeth.International journal of clinical pediatric dentistry · 2025Article
- Effects of Stress on Biological Characteristics and Metabolism of Periodontal Ligament Stem Cells of Deciduous Teeth.International dental journal · 2025Article
- Summed Tissue Resistance of Periodontal Ligaments and Alveolar Bone in Orthodontic Distal Retraction of Maxillary Canines: Mathematical Simulation of Clinical Data and Interpretation of Results.Dentistry journal · 2025Article
- Gene Polymorphism Influencing Persistent Bone Resorption in Brown Tumor: A Case Series with Possible Pathogenesis and Potential Therapeutic Approach.Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India · 2024Article
- Adult Mesenchymal Stem Cells from Oral Cavity and Surrounding Areas: Types and Biomedical Applications.Pharmaceutics · 2023Review
- Bioinductive and anti-inflammatory properties of Propolis and Biodentine on SHED.The Saudi dental journal · 2022Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
contextIt is a known fact that periodontal tissue regeneration can be achieved by the use of periodontal ligament stem cells (PDLSCs). Current mainstay of periodontal treatment is focusing on stem cell tissue engineering as an effective therapy, making it important to isolate PDLSCs from periodontal tissues.
aimsThe present research endeavor was undertaken to elucidate a technique for isolating PDLSCs for SETTINGS AND
designThe study design involves MATERIALS AND
methodsPremolar teeth were extracted from 12 patients who were under orthodontic treatment. PDL cells were scraped from their roots. Using 10 ml of Dulbecco's modified Eagle's medium with pH 7.2, the specimens of the periodontal tissue were transferred to laboratory where cell culture was done. Isolated stem cells were grown on 24-well microtiter plates-containing cover slips. They were incubated overnight at approximately 37°C in 95% air and 5% humidification. Anti-CD 45, CD73, CD90, CD105, and CD146 antibodies were used. After staining, cells were observed under phase-contrast microscopy and in inverted microscope.
resultsThe cells showed a marked growth and 90% confluence at day 6. Cells presented thin and long fibroblastic spindle morphology. Isolated PDLSCs showed colony-forming ability at the 14
conclusionsThe human PDLSCs can be clearly isolated and characterized by using CD90, CD73, CD146, and CD105 markers of stem cells.
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