Evidence map›Paper›PMID 34392450›Full record

ArticleMolecular biology reports2021

Development of nuclear and chloroplast polymorphic microsatellites for Crossostephium chinense (Asteraceae).

Luxian Liu, Shook Ling Low, Shota Sakaguchi, Yu Feng, Binjie Ge, Kamil Konowalik, Pan Li

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Factors affecting the genetic diversity ofFrontiers in plant science · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 6 institutions in 4 countries.

Luxian Liu *Key Laboratory of Plant Stress Biology, Laboratory of Plant Germplasm and Genetic Engineering, School of Life Sciences, Henan University, Kaifeng, 475000, China.
Shook Ling Low *University of Bonn, 53115, Bonn, Germany.
Shota SakaguchiDivision of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto, 6068502, Japan.
Yu FengLaboratory of Systematic & Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
Binjie GeEastern China Conservation Center for Wild Endangered Plant Resources, Shanghai Chenshan Botanical Garden, Shanghai, China.
Kamil KonowalikInstitute of Environmental Biology, Wrocław University of Environmental and Life Sciences, 51-631, Wrocław, Poland. kamil.konowalik@upwr.edu.pl.
Pan LiLaboratory of Systematic & Evolutionary Botany and Biodiversity, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China. panli_zju@126.com.ORCID http://orcid.org/0000-0002-9407-7740
Zhejiang University · CNHenan University · CNKyoto University · JPShanghai Chenshan Plant Science Research Center · CNUniversity of Bonn · DEWrocław University of Environmental and Life Sciences · PL

Funding

National Natural Science Foundation of China 31900188National Natural Science Foundation of China 31970225Natural Science Foundation of Zhejiang Province LY19C030007
6 · The paper itself

Abstract

backgroundCrossostephium chinense is a traditional Chinese medicinal herb and it is often cultivated as an ornamental plant. Previous studies on this species mainly focused on its chemical composition and it was rarely represented in genetic studies, and thus genomic resources remain scarce. METHODS AND

resultsBoth chloroplast and nuclear polymorphic microsatellites of C. chinense were screened from genome skimming data of two individuals. 64 and 63 cpSSR markers were identified from two chloroplast genomes of C. chinense. A total of 133 polymorphic nSSRs were developed. Ten nSSRs were randomly selected to test their transferability across 35 individuals from three populations of C. chinense, and 20 individuals each of Artemisia stolonifera and A. argyi. Cross-amplifications were successfully done for C. chinense and were partially amplified for both Artemisia species. The number of alleles varied from two to nine. The observed heterozygosity and expected heterozygosity per locus ranged from 0.000 to 0.286 and from 0.029 to 0.755, respectively.

conclusionsIn this study, we developed polymorphic cpSSRs and nSSRs markers for C. chinense based on genome skimming sequencing. These genomic resources will be valuable for population genetics and conservation studies in C. chinense and Artemisia.

Indexed as

Genome, ChloroplastPolymorphism, GeneticAllelesArtemisiaCell NucleusChloroplastsDNA, PlantDrugs, Chinese HerbalGenome, PlantHeterozygoteMicrosatellite RepeatsPhylogenyDNA, PlantDrugs, Chinese HerbalArtemisiaGenetic resourcesGenome skimmingMolecular markersSimple sequence repeats (SSRs)

Identifiers

PMID34392450
OpenAlexW3194901662

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.