Evidence map›Paper›PMID 34392397›Full record

ArticleDiabetologia2021

Normal to enhanced intrinsic mitochondrial respiration in skeletal muscle of middle- to older-aged women and men with uncomplicated type 1 diabetes.

Cynthia M F Monaco, Mark A Tarnopolsky, Athan G Dial, Joshua P Nederveen, Irena A Rebalka, Maria Nguyen, Lauren V Turner, Christopher G R Perry, Vladimir Ljubicic, Thomas J Hawke

Open access · bronzeAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in Diabetologia, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Cynthia M F MonacoDepartment of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.ORCID 0000-0003-2338-7714
Mark A TarnopolskyDepartment of Pediatrics, McMaster University, Hamilton, ON, Canada.ORCID 0000-0003-0312-3746
Athan G DialDepartment of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
Joshua P NederveenDepartment of Pediatrics, McMaster University, Hamilton, ON, Canada.
Irena A RebalkaDepartment of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.ORCID 0000-0002-4732-5473
Maria NguyenDepartment of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
Lauren V TurnerDepartment of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
Christopher G R PerrySchool of Kinesiology and Health Sciences, Muscle Health Research Centre, York University, Toronto, ON, Canada.ORCID 0000-0002-5745-5888
Vladimir LjubicicDepartment of Kinesiology, McMaster University, Hamilton, ON, Canada.ORCID 0000-0002-4592-4093
Thomas J HawkeDepartment of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada. hawke@mcmaster.ca.ORCID 0000-0003-4974-4820
McMaster University · CAYork University · CA

Funding

CIHR
6 · The paper itself

Abstract

aims/hypothesisThis study interrogated mitochondrial respiratory function and content in skeletal muscle biopsies of healthy adults between 30 and 72 years old with and without uncomplicated type 1 diabetes.

methodsParticipants (12 women/nine men) with type 1 diabetes (48 ± 11 years of age), without overt complications, were matched for age, sex, BMI and level of physical activity to participants without diabetes (control participants) (49 ± 12 years of age). Participants underwent a Bergström biopsy of the vastus lateralis to assess mitochondrial respiratory function using high-resolution respirometry and citrate synthase activity. Electron microscopy was used to quantify mitochondrial content and cristae (pixel) density.

resultsMean mitochondrial area density was 27% lower (p = 0.006) in participants with type 1 diabetes compared with control participants. This was largely due to smaller mitochondrial fragments in women with type 1 diabetes (-18%, p = 0.057), as opposed to a decrease in the total number of mitochondrial fragments in men with diabetes (-28%, p = 0.130). Mitochondrial respiratory measures, whether estimated per milligram of tissue (i.e. mass-specific) or normalised to area density (i.e. intrinsic mitochondrial function), differed between cohorts, and demonstrated sexual dimorphism. Mass-specific mitochondrial oxidative phosphorylation (OXPHOS) capacity with the substrates for complex I and complex II (C CONCLUSIONS/

interpretationIn middle- to older-aged adults with uncomplicated type 1 diabetes, we conclude that skeletal muscle mitochondria differentially adapt to type 1 diabetes and demonstrate sexual dimorphism. Importantly, these cellular alterations were significantly associated with our metric of aerobic fitness (PWC

Indexed as

AdultAgedCell RespirationDiabetes Mellitus, Type 1Electron Transport Complex IElectron Transport Complex IIFemaleHumansMaleMiddle AgedMitochondria, MuscleMuscle, SkeletalOxidative PhosphorylationOxygen ConsumptionRespiratory MechanicsElectron Transport Complex IElectron Transport Complex IIAerobic fitnessMitochondriaOlder adultsOxidative phosphorylationSkeletal muscleType 1 diabetes

Identifiers

PMID34392397
OpenAlexW3194572852

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.